Experimental infection of Peromyscus californicus ...



Title Experimental infection of Peromyscus californicus with Toxoplasma gondii
Author(s) H. A. Dabritz, M. A. Miller, A. E. Packham, D. Rejmanek, C. M. Leutenegger, I. A. Gardner, E. R. Atwill, P. A. Conrad
Journal Journal of Parasitology
Date 2007
Volume 93
Issue 6
Start page 1360
End page 1364
Abstract Eight female Peromyscus californicus were infected with 102 or 104 Toxoplasma gondii culture-derived tachyzoites (Type II or X) isolated from southern sea otters. All but 2 mice survived infection and developed antibodies to T. gondii. The 2 fatally infected mice were inoculated with 104 tachyzoites of the Type X strain. Parasite detection by immunohistochemistry (IHC) and DNA amplification with 2 polymerase chain reaction (PCR) methods was compared for brain, heart, lung, liver, spleen, biceps muscle, and tongue, at a mean of 41 days postinfection. Parasites were detected most commonly by IHC in spleen (8/8) and brain (6/8). DNA amplification by PCR was most successful from brain, heart, and spleen.
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