Lepeophtheirus salmonis secretory/excretory products ...

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Title Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation [electronic resource]
Author(s) M. D. FAST, D. PINTO, N. W. ROSS, S. C. JOHNSON, T. D. EDDY
Journal Parasite Immunology
Date 2007
Volume 29
Issue 4
Start page 179
End page 189
Abstract We have previously shown that Lepeophtheirus salmonis produces trypsin and prostaglandin E₂ (PGE₂) that are most likely responsible for the limited inflammatory response of Atlantic salmon to infection. After removal of the dopamine and PGE₂, the immunomodulatory activity of unfractionated and pools of the fractionated secretions was determined by examining the effects of the secretions on Atlantic salmon immune gene expression. Incubation of macrophage-enriched isolates of Atlantic salmon head kidney cells with the unfractionated secretion + PGE₂ revealed a significant inhibition of interleukin-1Îø (IL-1Îø) and major histocompatibility class I gene expression. Inhibition of lipopolysaccharide-induced IL-1Îø expression in the Atlantic salmon head kidney cell line (SHK-1) was observed when three pools of the secretory/excretory products were tested. Further purification of products within these pools revealed that fraction 1-2 could account fully for the inhibition of IL-1Îø expression in SHK-1 cells observed in pooled fraction 1. This study demonstrates that there are other immunomodulatory compounds produced by L. salmonis, in addition to PGE₂ and trypsin, that can inhibit the expression of Atlantic salmon immune-related genes in vitro.
DOI http://dx.doi.org/10.1111/j.1365-3024.2007.00932.x

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