An enzyme-based formaldehyde assay and its utility ...



Title An enzyme-based formaldehyde assay and its utility in a sponge sterol biosynthetic pathway
Author(s) R. G. Kerr, K. Kelly
Journal Journal of Natural Products
Date 1999
Volume 62
Issue 1
Start page 201
End page 202
Abstract An enzyme-based assay has been developed and utilized to confirm the production of formaldehyde in the dealkylation of sterol side chains in a marine sponge. The enzyme used in the assay, formaldehyde dehydrogenase, is NAD+ dependent, and the assay measures the production of NADH by determining the increase in fluorescence at 460 nm.
DOI 10.1021/np980366d

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