The sensitivity and specificity of a reverse ...
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| Title | The sensitivity and specificity of a reverse transcription-polymerase chain reaction assay for the avian pneumovirus (Colorado strain) |
| Author(s) | J. Pedersen, A. Ali, D. Reynolds |
| Journal | Avian Diseases |
| Date | 2000 |
| Volume | 44 |
| Issue | 3 |
| Start page | 681 |
| End page | 685 |
| Abstract | A reverse transcription-polymerase chain reaction (RT-PCR) assay for the detection of avian pneumovirus (APV), Colorado strain (US/CO), was evaluated for sensitivity and specificity. The single-tube RT-PCR assay utilized primers developed from the matrix (M) gene sequence of the US/CO APV. The RT-PCR amplified the US/CO APV but did not amplify other pneumoviruses, including the avian pneumoviruses subgroups A and B. The RT-PCR was capable of detecting between 10(0.25) mean tissue culture infective dose (TCID50) and 10(-0.44) TCID50 of the US/CO APV. These results have demonstrated that the single-tube RT-PCR assay is a specific and sensitive assay for the detection of US/CO APV. |
| ISSN | 00052086 |
Using APA 6th Edition citation style.
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