Isolation and serial propagation of turkey ...

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Title Isolation and serial propagation of turkey rotaviruses in a fetal rhesus monkey kidney (MA104) cell line
Author(s) K. W. Theil, D. L. Reynolds, Y. M. Saif
Journal Avian Diseases
Date 1986
Volume 30
Issue 1
Start page 93
End page 104
Abstract Turkey rotaviruses from the intestinal contents of poults were isolated and serially propagated in MA104 cell monolayers by a simple procedure. The initial virus isolation was done by low-speed centrifugation of the inoculum onto the monolayers, and subsequent passages were accomplished in roller-tube monolayers using trypsin-treated virus suspensions. Each of the turkey rotavirus isolates possessed the morphological, antigenic, and genomic attributes characteristic of turkey group A rotaviruses. Attempts to isolate and serially propagate turkey rotavirus-like viruses in MA104 cell monolayers by this procedure were unsuccessful. Turkey reoviruses also did not serially propagate in MA104 cell monolayers by this procedure.

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