Monensin use against Neospora caninum challenge in ...



Title Monensin use against Neospora caninum challenge in dairy cattle
Author(s) J. A. VanLeeuwen, S. Greenwood, F. Clark, A. Acorn, F. Markham, J. McCarron, R. O’Handley
Journal Veterinary Parasitology
Date 2011
Volume 175
Issue 3-4
Start page 372
End page 376
Abstract Using a randomized controlled trial design, a randomly allocated intervention group of 15 cows received a slow-release bolus that delivered 100 days of monensin. The negative control group of 15 cows received a placebo bolus that was identical to the monensin bolus, except without the monensin. Two weeks after bolus administration, all cows were challenged with a 2 ml subcutaneous injection of a live tachyzoite suspension. Whole blood and serum samples were collected from each cow every week for the first month post-challenge, and then every 2 weeks for the next 2 months. The extracted DNA from whole blood was tested for the Nc-5 gene fragment of Neospora caninum using a quantitative real time polymerase chain reaction. Serum was tested for antibodies to N. caninum using the IDEXX ELISA. Cows treated with monensin boluses had a significantly lower humoral immune response than cows treated with placebo boluses at one time point post-challenge (week 4 post-challenge). However, when adjusting for repeated measures within cows, the P value for this humoral difference was 0.098. No DNA for N. caninum was detected in either group, likely due to study design features.
DOI 10.1016/j.vetpar.2010.10.016

Using APA 6th Edition citation style.

[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]

Times viewed: 771

Adding this citation to "My List" will allow you to export this citation in other styles.