Monensin use against Neospora caninum challenge in ...
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| Title | Monensin use against Neospora caninum challenge in dairy cattle |
| Author(s) | J. VanLeeuwen, S. Greenwood, F. Clark, A. Acorn, F. Markham, J. McCarron, R. O’Handley |
| Journal | Veterinary Parasitology |
| Date | 2011 |
| Volume | 175 |
| Issue | 3-4 |
| Start page | 372 |
| End page | 376 |
| Abstract | Using a randomized controlled trial design, a randomly allocated intervention group of 15 cows received a slow-release bolus that delivered 100 days of monensin. The negative control group of 15 cows received a placebo bolus that was identical to the monensin bolus, except without the monensin. Two weeks after bolus administration, all cows were challenged with a 2 ml subcutaneous injection of a live tachyzoite suspension. Whole blood and serum samples were collected from each cow every week for the first month post-challenge, and then every 2 weeks for the next 2 months. The extracted DNA from whole blood was tested for the Nc-5 gene fragment of Neospora caninum using a quantitative real time polymerase chain reaction. Serum was tested for antibodies to N. caninum using the IDEXX ELISA. Cows treated with monensin boluses had a significantly lower humoral immune response than cows treated with placebo boluses at one time point post-challenge (week 4 post-challenge). However, when adjusting for repeated measures within cows, the P value for this humoral difference was 0.098. No DNA for N. caninum was detected in either group, likely due to study design features. |
| DOI | 10.1016/j.vetpar.2010.10.016 |
Using APA 6th Edition citation style.
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