Method development and generation of profiles for ...



Title Method development and generation of profiles for selected phenolics from apple cultivars used for processed products
Author(s) L. F. Russell, L. I. Wolfe, D. A. J. Ryan
Journal Journal of Food Science
Date 2002
Volume 67
Issue 8
Start page 2819
End page 2826
Abstract Profiles of selected phenolic constituents of 2 apple cultivars, Northern Spy and Ida Red, commonly used for processed products Were compared. Although chlorogenic acid was the principal phenolic in both cultivars, there were significant quantitative differences. A new solid-phase extraction (SPE) and high-performance liquid chromatographic (HPLC) separation using polymeric resins was developed for this experiment. Quantification was by ultra-violet (UV) absorbance using a diode array detector. The performance of this new method was compared with that of a published liquid-liquid extraction (LLE) method, based on linearity of detector response, limits of detection, reproducibility of analysis, and recoveries from spiked apple samples.

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