Dimethyl-acetamide as a cryoprotectant for rainbow ...
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Citation
| Title | Dimethyl-acetamide as a cryoprotectant for rainbow trout spermatozoa |
| Author(s) | M. McNiven, R. Gallant, G. Richardson |
| Journal | Theriogenology |
| Date | 1993 |
| Volume | 40 |
| Issue | 5 |
| Start page | 943 |
| End page | 948 |
| Abstract | Dimethyl-acetamide was evaluated as a cryoprotectant for rainbow trout (Oncorhynchus mykiss ) semen on the basis of motility, percentage of dead spermatozoa as determined by fluorometry, and fertility of frozen-thawed spermatozoa. Dimethyl-acetamide performed significantly better (P<0.05) than the conventional cryoprotectant, dimethyl sulfoxide, by all evaluation methods. An extender comprised of 0.137 M NaCl, 0.011 M KCl, 0.004 M Na(2)HPO(4) 7H(2)O, 7.5 g/l L-alpha-lecithin and 10 % DMA showed promise for cryopreserving rainbow trout spermatozoa. Future evaluation of new extenders should be carried out by several in vitro techniques and fertility measurements to present a complete assessment of an extender's ability to cryopreserve sperm cells. |
| ISSN | 0093-691X |
Using APA 6th Edition citation style.
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