Development of a TaqMan PCR assay for the detection ...

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Title Development of a TaqMan PCR assay for the detection of Bonamia species
Author(s) S. Corbeil, I. Arzul, B. Diggles, M. Heasman, B. Chollet, F. C. J. Berthe, M. S. Crane
Journal Diseases of Aquatic Organisms
Date 2006
Volume 71
Issue 1
Start page 75
End page 80
Abstract The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detection of Bonamia species in infected oyster tissues. The TaqMan assay was shown to be significantly more sensitive than histopathology. Although a real-time TaqMan PCR assay is comparable with conventional PCR in terms of sensitivity, it offers the advantages that it is a rapid test and has a very low risk of sample cross-contamination. Furthermore, it can be optimised to quantify the parasite load in samples. The assay detected Bonamia isolates from Australia, New Zealand, Europe, Canada, Chile and the USA and therefore demonstrated genus specificity as tested in this study.
DOI 10.3354/dao071075
PubMed ID 16922002
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