Evaluation of an automated colorimetric assay for ...
|Title||Evaluation of an automated colorimetric assay for the measurement of lipase activity in canine sera|
|Author(s)||A. L. Mackenzie, Shelley A. Burton, D. W. Olexson, Barbara S. Horney, D. J. Honor, P. B. Maloney, S. D. Buczkowski|
|Journal||Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Veterinaire|
|Abstract||An automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by pancreatic lipase. Within-run and between-run coefficients of variation were 7 y) dogs using this assay. It was concluded that the automated colorimetric assay was a reliable indicator of canine serum lipase activity and offered several advantages, including small sample volume and short analysis time.|
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