Evaluation of an automated colorimetric assay for ...
|Title||Evaluation of an automated colorimetric assay for the measurement of lipase activity in canine sera|
|Author(s)||A. Mackenzie, S. Burton, D. Olexson, B. Horney, D. Honor, P. Maloney, S. Buczkowski|
|Journal||Canadian journal of veterinary research = Revue canadienne de recherche veterinaire|
|Abstract||An automated colorimetric method for determining lipase activity in canine sera was evaluated for precision, linearity and correlation to existing assay methods. The colorimetric method was a commercial reagent that used a series of enzymatic reactions based on the hydrolysis of 1,2 diglyceride by pancreatic lipase. Within-run and between-run coefficients of variation were 7 y) dogs using this assay. It was concluded that the automated colorimetric assay was a reliable indicator of canine serum lipase activity and offered several advantages, including small sample volume and short analysis time.|
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