In vitro cultivation of the vascular phase of ...

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Title In vitro cultivation of the vascular phase of Sarcocystis capracanis and Sarcocystis tenella
Author(s) C. A. Speer, Richard J. Cawthorn, J. P. Dubey
Journal The Journal of Protozoology
Date 1986
Volume 33
Issue 4
Start page 486
End page 490
Abstract Sporozoites of Sarcocystis capracanis and S. tenella (Apicomplexa) penetrated all four cell types tested (bovine monocytes, BM; bovine pulmonary artery endothelial cells, CPA; Madin-Darby bovine kidney; and ovine monocytes). Sporozoites of S. tenella developed to meronts in BM and CPA; those of S. capracanis developed to meronts in BM only. Both species of Sarcocystis developed to large first-generation meronts followed by small meronts. At 40 to 50 days after inoculation (DAI) of sporozoites, considerably more merozoites of S. tenella were harvested from CPA (24.9 X 10(6) merozoites/75-cm2 flask; n = 4) than from BM (1.9 X 10(6) merozoites/75-cm2 flask; n = 4). Merozoites of S. capracanis were most numerous in BM at 88 to 100 DAI during which time 2.1 X 10(6) merozoites/75-cm2 flask (n = 4) were harvested.

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