Mushroom tyrosinase as a control material for ...
|Title||Mushroom tyrosinase as a control material for phenoloxidase assays used in the assessment of crustacean "health"|
|Author(s)||A. Battison, Richard J. Cawthorn, Barbara S. Horney, A. Mackenzie|
|Journal||Journal of Shellfish Research|
|Abstract||Mushroom tyrosinase (E.C. 220.127.116.11) was evaluated as a control material for use in a plasma prophenoloxidase assay in the American lobster Homarus americanus as part of the assay's validation procedure. Reconstituted. lyophilized tyrosinase solution was stable for 72 h at 2-4degreesC. Percent recoveries were 94%. 120%, 113%, and 48% for "mid-range" activity dilutions and 93%, 94%, 87%. and 38% for "low-range" activity dilutions at 24, 48, 72, and 96 h, respectively. The activity of the reconstituted tyrosinase solution was stable for 4 wk when stored at -80degreesC, and percentage recoveries, compared to activity of the freshly reconstituted solution, were generally within 15% for both mid-range and "high"-activity dilutions. The between-run coefficients of variation (CVs) were 10.6% for the mid-range and 10.8% for the high-activity dilution over the 4-wk period. Enzyme activity was unstable when stored at -20degreesC. Mushroom tyrosinase is an acceptable control material for use in assays of phenoloxidase activity. Inclusion of this control material will provide a means to confidently compare results on a day-to-day or run-to-run basis when phenoloxidase assays are used in the assessment of crustacean "health.".|
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