Mushroom tyrosinase as a control material for ...

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Title Mushroom tyrosinase as a control material for phenoloxidase assays used in the assessment of crustacean "health"
Author(s) A. Battison, Richard J. Cawthorn, Barbara S. Horney, A. Mackenzie
Journal Journal of Shellfish Research
Date 2002
Volume 21
Issue 1
Start page 295
End page 298
Abstract Mushroom tyrosinase (E.C. 1.14.18.1) was evaluated as a control material for use in a plasma prophenoloxidase assay in the American lobster Homarus americanus as part of the assay's validation procedure. Reconstituted. lyophilized tyrosinase solution was stable for 72 h at 2-4degreesC. Percent recoveries were 94%. 120%, 113%, and 48% for "mid-range" activity dilutions and 93%, 94%, 87%. and 38% for "low-range" activity dilutions at 24, 48, 72, and 96 h, respectively. The activity of the reconstituted tyrosinase solution was stable for 4 wk when stored at -80degreesC, and percentage recoveries, compared to activity of the freshly reconstituted solution, were generally within 15% for both mid-range and "high"-activity dilutions. The between-run coefficients of variation (CVs) were 10.6% for the mid-range and 10.8% for the high-activity dilution over the 4-wk period. Enzyme activity was unstable when stored at -20degreesC. Mushroom tyrosinase is an acceptable control material for use in assays of phenoloxidase activity. Inclusion of this control material will provide a means to confidently compare results on a day-to-day or run-to-run basis when phenoloxidase assays are used in the assessment of crustacean "health.".
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