Molecular characterization and substrate specificity ...
|Title||Molecular characterization and substrate specificity of nitrobenzene dioxygenase from comamonas sp. strain JS765|
|Author(s)||D. J. Lessner, Gerald R. Johnson, R. E. Parales, J. C. Spain, D. T. Gibson|
|Journal||Applied and Environmental Microbiology|
|Abstract||Comamonas sp. strain JS765 can grow with nitrobenzene as the sole source of carbon, nitrogen, and energy. We report here the sequence of the genes encoding nitrobenzene dioxygenase (NBDO), which catalyzes the first step in the degradation of nitrobenzene by strain JS765. The components of NBDO were designated Reductase sub(NBZ), Ferredoxin sub(NBZ), Oxygenase sub(NBZ alpha ), and Oxygenase sub(NBZs), with the gene designations nbzAa, nbzAb, nbzAc, and nbzAd, respectively. Sequence analysis showed that the components of NBDO have a high level of homology with the naphthalene family of Rieske nonheme iron oxygenases, in particular, 2-nitrotoluene dioxygenase from Pseudomonas sp. strain JS42. The enzyme oxidizes a wide range of substrates, and relative reaction rates with partially purified Oxygenase sub(NBZ) revealed a preference for 3-nitrotoluene, which was shown to be a growth substrate for JS765. NBDO is the first member of the naphthalene family of Rieske nonheme iron oxygenases reported to oxidize all of the isomers of mono- and dinitrotoluenes with the concomitant release of nitrite.|
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