Formation of virus-like particles when the ...



Title Formation of virus-like particles when the polyprotein gene (segment A) of infectious bursal disease virus is expressed in insect cells
Author(s) Frederick S. B. Kibenge, B. Qian, E. Nagy, J. R. Cleghorn, D. Wadowska
Journal Canadian Journal of Veterinary Research = Revue Canadienne de Recherche Veterinaire
Date 1999
Volume 63
Issue 1
Start page 49
End page 55
Abstract The baculovirus expression vector system was used to examine the expression of the full-length infectious bursal disease virus (IBDV) segment A cDNA, which encodes the structural proteins in a polyprotein precursor that is autocatalytically cleaved to VPX, VP3, and VP4. No VP2 was observed in lysates of recombinant baculovirus infected cells indicating the lack of processing of VPX to VP2 in this system. Virus-like particles (VLP) were purified from the infected insect cells, and on negative staining electron microscopy, looked very similar to authentic IBDV particles in shape and size, suggesting that processing of VPX to VP2 is not necessary for capsid assembly.

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