A rapid radioimmunoassay method for the evaluation ...



Title A rapid radioimmunoassay method for the evaluation of plasma cortisol levels and adrenal function in the dog
Author(s) S. J. Lester, James E. C. Bellamy, P. S. MacWilliams, E. C. Feldman
Journal Journal of the American Animal Hospital Association
Date 1981
Volume 17
Issue 1
Start page 121
End page 128
Abstract A rapid radioimmunoassay method for detecting plasma cortisol was evaluated and the results found to be accurate, precise and reproducible. Sample storage and preparation were examined; refrigeration and delayed serum separation decreased the cortisol concentration but freezing for up to six months had no detectable effect on the cortisol level. Plasma cortisol levels were evaluated to determine their usefulness in diagnosing primary adrenal dysfunction in the dog. Neither basal cortisol levels nor the incremental rise in cortisol after adrenocorticotropin (ACTH) injection completely distinguished between normal dogs and those with adrenal disease. However, in the dogs which responded to ACTH, the cortisol levels sixty minutes after its injection provided unambiguous differentiation of those animals with adrenal disease..

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