Application of pulsed-field gel electrophoresis and ...



Title Application of pulsed-field gel electrophoresis and binary typing as tools in veterinary clinical microbiology and molecular epidemiologic analysis of bovine and human Staphylococcus aureus isolates
Author(s) R. Zadoks, W. van Leeuwen, H. W. Barkema, O. Sampimon, H. Verbrugh, Y. H. Schukken, A. van Belkum
Journal Journal of Clinical Microbiology
Date 2000
Volume 38
Issue 5
Start page 1931
End page 1939
Abstract 38 bovine mammary Staphylococcus aureus isolates from diverse clinical, temporal and geographical origins were genotyped by pulsed-field gel electrophoresis (PFGE) after SmaI digestion of prokaryotic DNA and by means of binary typing using 15 strain-specific DNA probes. Seven pulsed-field types and 4 subtypes were identified, as were 16 binary types. Concordant delineation of genetic relatedness was documented by both techniques, yet based on practical and epidemiological considerations, binary typing was the preferable method. Genotypes of bovine isolates were compared to 55 previously characterized human S. aureus isolates through cluster analysis of binary types. Genetic clusters containing strains of both human and bovine origin were found, but bacterial genotypes were predominantly associated with a single host species. Binary typing proved an excellent tool for comparison of S. aureus strains, including methicillin-resistant S. aureus, derived from different host species and from different databases. For 28 bovine S. aureus isolates, detailed clinical observations in vivo were compared to strain typing results in vitro. Associations were found between distinct genotypes and severity of disease, suggesting strain-specific bacterial virulence. It is concluded that PFGE and binary typing can be successfully applied for genetic analysis of S. aureus isolates from bovine mammary secretions. Binary typing in particular is a useful and simple method for strain characterization, for resolution of clonal relationships of bacteria within and between host species and for identification of sources and transmission routes of bovine S. aureus..

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