Cryopreservation of Arctic charr, Salvelinus alpinus ...

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Title Cryopreservation of Arctic charr, Salvelinus alpinus (L.), semen in various extenders and in three sizes of straw
Author(s) Gavin F. Richardson, T. L. Miller, Mary A. McNiven
Journal Aquaculture Research
Date 2000
Volume 31
Issue 3
Start page 307
End page 315
Abstract Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA) or 20% glycerol] and 3 sizes of straw. The 3 diluents and 3 cryoprotectants were combined, resulting in 9 extenders. One part semen was added to 3 parts extender, and motility was evaluated to assess the toxicity of 6 of the extenders. Semen in 9 extenders was frozen in 0.5-ml straws using liquid nitrogen vapour. Semen extended in 0.3 M glucose and each of the cryoprotectants was frozen in 0.5-ml, 1.7-ml (flat) or 2.5-ml straws. The freezing rate in each size of straw was measured. Fertility trials were conducted to determine the post-thaw viability of the frozen semen. The motility of activated spermatozoa was higher in the DMA and DMSO extenders than in the glycerol extender. For the trial using 0.5-ml straws, post-thaw fertility results were higher for all extenders containing DMSO, or 0.3 M glucose and DMA, than for all other combinations of diluent and cryoprotectant. For the straw size comparison, the highest fertility was obtained with 1.7-ml straws using either DMSO or DMA and with 2.5-ml straws using DMSO. For all cryopreservation trials, fertility was low for extenders containing glycerol..

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