Lepeophtheirus salmonis secretory/excretory products ...
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| Title | Lepeophtheirus salmonis secretory/excretory products and their effects on Atlantic salmon immune gene regulation [electronic resource] |
| Author(s) | M. FAST, D. PINTO, N. ROSS, S. JOHNSON, T. EDDY |
| Journal | Parasite Immunology |
| Date | 2007 |
| Volume | 29 |
| Issue | 4 |
| Start page | 179 |
| End page | 189 |
| Abstract | We have previously shown that Lepeophtheirus salmonis produces trypsin and prostaglandin E₂ (PGE₂) that are most likely responsible for the limited inflammatory response of Atlantic salmon to infection. After removal of the dopamine and PGE₂, the immunomodulatory activity of unfractionated and pools of the fractionated secretions was determined by examining the effects of the secretions on Atlantic salmon immune gene expression. Incubation of macrophage-enriched isolates of Atlantic salmon head kidney cells with the unfractionated secretion + PGE₂ revealed a significant inhibition of interleukin-1Îø (IL-1Îø) and major histocompatibility class I gene expression. Inhibition of lipopolysaccharide-induced IL-1Îø expression in the Atlantic salmon head kidney cell line (SHK-1) was observed when three pools of the secretory/excretory products were tested. Further purification of products within these pools revealed that fraction 1-2 could account fully for the inhibition of IL-1Îø expression in SHK-1 cells observed in pooled fraction 1. This study demonstrates that there are other immunomodulatory compounds produced by L. salmonis, in addition to PGE₂ and trypsin, that can inhibit the expression of Atlantic salmon immune-related genes in vitro. |
| DOI | http://dx.doi.org/10.1111/j.1365-3024.2007.00932.x |
| ISSN | 01419838 |
Using APA 6th Edition citation style.
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