Mushroom tyrosinase as a control material for ...
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| Title | Mushroom tyrosinase as a control material for phenoloxidase assays used in the assessment of crustacean "health" |
| Author(s) | A. Battison, R. Cawthorn, B. Horney, A. Mackenzie |
| Journal | Journal of Shellfish Research |
| Date | 2002 |
| Volume | 21 |
| Issue | 1 |
| Start page | 295 |
| End page | 298 |
| Abstract | Mushroom tyrosinase (E.C. 1.14.18.1) was evaluated as a control material for use in a plasma prophenoloxidase assay in the American lobster Homarus americanus as part of the assay's validation procedure. Reconstituted. lyophilized tyrosinase solution was stable for 72 h at 2-4degreesC. Percent recoveries were 94%. 120%, 113%, and 48% for "mid-range" activity dilutions and 93%, 94%, 87%. and 38% for "low-range" activity dilutions at 24, 48, 72, and 96 h, respectively. The activity of the reconstituted tyrosinase solution was stable for 4 wk when stored at -80degreesC, and percentage recoveries, compared to activity of the freshly reconstituted solution, were generally within 15% for both mid-range and "high"-activity dilutions. The between-run coefficients of variation (CVs) were 10.6% for the mid-range and 10.8% for the high-activity dilution over the 4-wk period. Enzyme activity was unstable when stored at -20degreesC. Mushroom tyrosinase is an acceptable control material for use in assays of phenoloxidase activity. Inclusion of this control material will provide a means to confidently compare results on a day-to-day or run-to-run basis when phenoloxidase assays are used in the assessment of crustacean "health.". |
| ISSN | 0730-8000 |
| Use/Reproduction | Contact Author Contact Publisher |
| Text | http://www.islandscholar.ca/download_ds/ir:ir-batch6-3450/PDF |
Using APA 6th Edition citation style.
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