The superoxide dismutases of Bacillus anthracis do ...
|Title||The superoxide dismutases of Bacillus anthracis do not cooperatively protect against endogenous superoxide stress|
|Author(s)||K. Passalacqua, N. Bergman, A. Herring Palmer, P. Hanna|
|Journal||Journal of Bacteriology|
|Abstract||The Bacillus anthracis chromosome encodes four unique, putative superoxide dismutase (sod) genes. During exponential growth and sporulation, sodA1, sodA2, and sodC are transcribed constitutively throughout the growth cycle as individual genes. In contrast, the transcription of sod15 occurs mainly during late exponential and sporulation phases as part of a four-gene operon that may be involved in spore formation. Vegetative cell and spore lysates of wild-type Sterne and superoxide dismutase deletion ( Delta sod) mutants show detectable SOD activity for SODA1 and SODA2, and protein analysis suggests that these two proteins form active homodimers and heterodimers. A comparison of the growth of parental versus Delta sod mutants under various chemical oxidative stresses indicates that Delta sodA1 mutants are particularly sensitive to endogenously produced superoxide, whereas Delta sodA2, Delta sod15, and Delta sodC mutants remain as resistant to this stress as the parental strain. In addition, in mouse survival assays, Delta sod15 and Delta sodA1 were responsible for less end-point death, but the level of decreased virulence does not fall within a statistically significant range. Collectively, these data show that sodA1 acts as a major protectant from intracellular superoxide stress, that sod15 is transcribed as part of an operon that may play a role in cell morphology, and that sodA2 and sodC may have minor roles that are not apparent in the conditions tested here..|
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