Electrophoretic and immunochemical analyses of the ...
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Citation
| Title | Electrophoretic and immunochemical analyses of the lipopolysaccharides from various strains of Aeromonas hydrophila |
| Author(s) | J. Dooley, R. Lallier, D. Shaw, T. Trust |
| Journal | Journal of Bacteriology |
| Date | 1985 |
| Volume | 164 |
| Issue | 1 |
| Start page | 263 |
| End page | 269 |
| Abstract | Lipopolysaccharides isolated from strains of Aeromonas hydrophila, virulent for fish and which autoaggregate during growth in static broth culture, were examined by sodium dodecyl sulphate-polyacrylamide gel electrophoresis. The lipopolysaccharides contained O-polysaccharide chains of homogeneous chain length. Two of the strains produced a surface protein array, and immunofluorescence and phage-binding showed that a number of these O-polysaccharide chains of homogeneous length traversed the protein array and were exposed on the cell surface. There were three epitopes on the polysaccharide portion of this homogenous-chain-length lipopolysaccharide morphotype. One epitope was species serogroup specific and reactive by immunoblotting. This epitope was not present on the heterogeneous-chain-length O polysaccharides of non-autoaggregating strains of A. hydrophia examined. The second epitope was conformation dependent and cross-reactive with an epitope on the homogenous-chain-length O polysaccharides of Aeromonas salmonicida lipopolysaccharide. The third epitope was recognized by a monoclonal antibody and appeared to involve that region of the A. hydrophila and A. salmonicida lipopolysaccharide molecules which contained the O-polysaccharide-core oligosaccharide glycosidic linkage.. |
| ISSN | 0021-9193 |
Using APA 6th Edition citation style.
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