Feulgen Iron Propiono Carmine and Cupra Ammonium in ...
Description
Citation
| Title | Feulgen Iron Propiono Carmine and Cupra Ammonium in Preparing Algal Chromosomes for Light Microscopy |
| Author(s) | L. Hanic |
| Journal | Stain technology |
| Date | 1979 |
| Volume | 54 |
| Issue | 3 |
| Start page | 129 |
| End page | 134 |
| Abstract | A method for obtaining algal chromosomal preparations is described employing the Fuelgen method for DNA staining, Fe-propionocarmine as an enhancing stain, and cupra-ammonium to remove cell wall material. Fe-propionocarmine applied as a gradient to the slide provides cells stained with the Feulgen stain alone or with the Fuelgen Fe-propionocarmine stain, thereby facilitating useful comparison. Where dilute, the Fe-propionocarmine enhances nuclear staining without staining other organelles; where more concentrated, it also stains the nucleolus, spindle, spindle polar bodies, pyrenoid and protoplast. Treatment with cupra-ammonium, to remove polysaccharide wall material, followed by neutralization with propionocarmine, enables thinner squashes and better chromosome spreads without loss of differential staining. Preparations mounted in euparal are long-lasting. |
| ISSN | 0038-9153 |
Using APA 6th Edition citation style.
[Page generation failure. The bibliography processor requires a browser with Javascript enabled.]
Times viewed: 80
