Use of a microplate reader in an assay of ...
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| Title | Use of a microplate reader in an assay of glutathione reductase using 5,5'-dithiobis(2-nitrobenzoic acid) |
| Author(s) | A. Cribb, J. Leeder, S. Spielberg |
| Journal | Analytical Biochemistry |
| Date | 1989 |
| Volume | 183 |
| Issue | 1 |
| Start page | 195 |
| End page | 196 |
| Abstract | The use of 96-well microtiter plates and a programmable microplate reader to measure glutathione reductase in an assay based on reduction of 5,5'-dithiobis(2-nitrobenzoic acid) by GSH generated from an excess of GSSG is described. Samples are prepared in 96-well plates and absorbance at 415 nm with a reference wavelength of 595 is determined every 30 s for 3 min. The rate of increase in absorbance is directly proportional to the amount of glutathione reductase in the sample. Activity in an unknown sample is determined from a standard curve. The assay is rapid and allows many small samples to be analyzed in replicates of two or more at the same time. |
| ISSN | 0003-2697 |
Using APA 6th Edition citation style.
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