A reverse transcription-polymerase chain reaction assay for the detection of avian pneumovirus (Colorado strain)
Ali, A.Reynolds, D. L.
Pathology and Microbiology
A reverse transcription-polymerase chain reaction assay was developed to detect of avian pneumovirus (Colorado strain) (APV-Col). The specific primers were designed from the published sequence of the matrix protein gene of APV-Col. The primers amplified a product of 631 nucleotides from APV-Col. The assay identified only APV-Col and did not react with Newcastle disease virus or infectious bronchitis virus.