An enzyme-based formaldehyde assay and its utility in a sponge sterol biosynthetic pathway
Kerr, R. G.Kelly, K.
Journal of Natural Products
An enzyme-based assay has been developed and utilized to confirm the production of formaldehyde in the dealkylation of sterol side chains in a marine sponge. The enzyme used in the assay, formaldehyde dehydrogenase, is NAD+ dependent, and the assay measures the production of NADH by determining the increase in fluorescence at 460 nm.