Effects of a pathogenic canine herpesvirus on canine brain cell cultures and cerebellar expiants
Johnson, Gerald R.Koestner, A.Kindig, O.Long, J. F.
Pathology and Microbiology
Examination over a period of 72 hours from inoculation showed that lesions developed first in astrocytes. where nucleolar changes. the appearance of three types of inclusion bodies, virus antigen and virus particles preceded cell degeneration and virus release. Less numerous inclusions were found in oligodendrocytes, but none in neurones, although these were irreversibly damaged. The in-vitro results obtained agree closely with other in-vivo studies on the course of the disease.-MRC..