Primers and DNA probes designed for use in the specific detection of the paramyxean parasites Marteilia sydneyi and Marteilia refringens were tested for their potential to cross-react with closely related species in Polymerase Chain Reaction (PCR) and in situ hybridization. PCR primers and a DNA pro...

Piscirickettsia salmonis, the etiologic agent of salmonid rickettsial septicemia (SRS), or piscirickettsiosis, causes substantial economic losses to the salmon industry. The pathogenesis of the disease has not been fully characterized. The aim of this study is to describe the hepatic lesions associa...

The early ultrastructural stages of Loma salmonae were studied in the gills of experimentally infected rainbow trout. No parasitic stages were identified during the first 2 wk of the infection. By week 3 postexposure (PE), uninucleate and binucleate meronts were recognized within host cells (no xeno...

Two groups of healthy chinook salmon (Oncorhynchus tshawytscha) were experimentally exposed to gas supersaturated groundwater. Gross lesions consistent with gas bubble disease (GBD) developed. Vascular lesions associated with intravascular gas bubbles were examined with light and scanning electron m...

A massive fish kill affecting exclusively common carp (Cyprinus carpio carpio) in the St. Lawrence River, Quebec, Canada, during the summer of 2001 was investigated by use of laboratory diagnostic methods and by an attempt to experimentally induce the disease. The ultimate causes of mortality were o...

Hyperthyroidism can increase the renal excretion of magnesium and thus cause hypomagnesemia in various species. Anaerobically collected blood samples from 15 hyperthyroid and 40 normal, healthy cats were analyzed with an ion-selective electrode analyzer and a serum biochemical analyzer. There was no...

Five cases of hypoadrenocorticism in young related Nova Scotia duck tolling retrievers are discussed. Two were littermates and the others shared one or more common ancestors. No specific mode of inheritance was determined. Clinical and laboratory findings typical of hypoadrenocorticism were observed...

Antibodies to seven antigens in a whole cell lysate of Corynebacterium pseudotuberculosis ranging in molecular mass from 22 to 120 kilodaltons (kDa) were present in sera of 40 sheep and goats infected with C. pseudotuberculosis. Three antigens of about 120, 68, and 31.5 kDa in size were consistently...

Histology and immunohistochemistry were used to analyse the lesions and distribution of Mycoplasma bovis antigen in the lungs of 18 naturally infected calves. Microscopic examination of pneumonic lungs revealed two distinct patterns of necrosis and inflammation. The first pattern was observed in six...

Non-typhoid Salmonella serovars remain a potential threat to human health, and beef cattle and broiler chickens are possible sources of these organisms on Prince Edward Island (PEI). In this study, the ceca of beef cattle belonging to fasted and non-fasted groups, and broiler chickens were examined ...

Life-span and reproductive capacity of sea lice, Lepeophtheirus salmonis, were investigated in artificial seawater (Instant OceanReg.) under laboratory conditions. Sea lice were maintained on Atlantic salmon (Salmo salar) in recirculation systems for as long as 7 months. During this period sea lice ...

"Meconium aspiration syndrome" is a condition resulting in respiratory distress and the occasional death of newborn human beings. A retrospective study was conducted on 52 calves that were submitted for postmortem examination to the Atlantic Veterinary College, Charlottetown, Prince Edward...

Interaction of native proteinaceous antigens during the recognition and the effector phases of an immune response leads to antigenic conformational modifications which may elicit additional specific immune response. Protein cross-linking and conformation restraining formaldehyde and glutaraldehyde h...

Infectious salmon anaemia (ISA) virus (ISAV) is currently one of the most important viral pathogens threatening commercial aquaculture in the northern hemisphere. The virus is classified in the family Orthomyxoviridae, genus Isavirus. The virus offers a significant challenge to fish biologists inter...

Walleye, (Stizostedion vitreum), from two northern Minnesota, USA spawning stations (Pike River, Hudson Bay drainage, and Little Cutfoot Sioux Lake, Mississippi River drainage) simultaneously stocked as fry into five southern Minnesota lakes had different survival rates. One year after stocking, Pik...

A specific DNA probe for the identification of Renibacterium salmoninarum, the causative agent of bacterial kidney disease (BKD), was developed from one of 3 clones pRS47, pRS49, and pRS26 of 5.1 kb, 5.3 kb, and 11.3 kb, respectively. The biotinylated pRS47/BamHI insert probe was tested on 3 dilutio...

The factors affecting the horizontal transmission of infectious salmon anaemia virus (ISAV) among naive Atlantic salmon Salmo salar maintained in freshwater were examined. The ratio of injected to naive cohabitants was varied and mortality was monitored. Whether ISAV transmission in freshwater requi...

In the autumn of 2001, a self-administered mailed questionnaire was sent to fin fish recirculation facilities in the United States and Canada to examine the extent of biosecurity currently practiced in this sector of aquaculture. This paper describes the results of this survey (86% response rate). I...

Free sporocysts of a Sarcocystis-like organism (11.6 x 9.2 micro m with a diffuse residuum of numerous small refractile granules) from intestinal scrapings of an N. scandiaca from Saskatchewan, Canada, were orally administered to laboratory-reared D. richardsoni. Merants were present in hepatocytes ...