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1426 - 1450 of 1625
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Two cases of Amoeba-like infections in cultured warmwater marine fish are described, a systemic infection in pompano T. falcatus from Singapore and a gill infection in Mediterranean (Greece) sea bream S. auratus. All pompano showed marked systemic infection of Amoeba-like parasites in the gills, kid...
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A colorimetric assay employing MTT (3-[4,5-dimethylthiazole-2-yl], 2-5-diphenyltetrazolium bromide) was used to determine the mitogenic response of intestinal intraepithelial lymphocytes (i-IELs) of chickens to T- and B-cell mitogens. Comparisons between mitogenic responses of i-IELs and peripheral ...
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Mitogenic responsiveness of intestinal intraepithelial lymphocytes (i-IEL) to concanavalin A (Con A), phytohemagglutinin P (PHA-P), and lipopolysaccharide (LPS) from Salmonella typhimurium were evaluated in various age groups of turkeys by a colorimetric blastogenic microassay. Comparisons were made...
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A field trial was conducted to measure differences in performance between selenium-supplemented and nonsupplemented heifers on a 1200-cow California dairy. 117 19- to 27-month-old Holstein heifers were randomly assigned to treatment (n = 59) and control (n = 58) groups. A federally approved, commerc...
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A rotavirus isolated from the intestinal contents of commercial ring-neck pheasant chicks was identified as a group D rotavirus based on its RNA electropherotype and its serological relatedness to known turkey group A and D rotaviruses using immune electron microscopy and agar-gel immunodiffusion as...
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A dot-immunobinding assay was enhanced by the incorporation of avidin and biotin reagents into the test system (DAB assay). This assay was used to detect serum antibodies to Mycoplasma gallisepticum (NG) and M. synoviae (MS) from chickens. Serum samples were tested by rapid serum plate (RSP), haemag...
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A dot-immunobinding assay, amplified with avidin and biotin (DAB assay), was used to detect serum antibodies to Mycoplasma iowae in immunized turkeys. The DAB assay was used to test serum samples from 122 commercial market turkey flocks obtained from four Iowa processing plants. The samples were poo...
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The intestinal tracts of ringneck pheasant chicks inoculated with group D rotavirus at 8 days old, were examined PM by light and electron microscopy at 4, 7 or 11 after inoculation. After 4 days, 2 (66%) of 3 birds were stunted and had diarrhoea and dilated intestines. Intestinal villi were shortene...
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CmeABC, a multidrug efflux system in Campylobacter jejuni, plays an important role in the resistance to different antimicrobials and toxic compounds. Although this efflux system has been well characterized in C. jejuni and to a less extent in C. coli, it is unknown if CmeABC homologs are functional ...
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Lepeophtheirus salmonis is an ectoparasitic copepod that causes serious disease outbreaks in both wild and farmed salmonids. As the relationship between L. salmonis and its hosts is not well understood, the current investigation was undertaken to investigate whether any immunomodulatory compounds co...
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Some bacteria can communicate with other species of bacteria by means of autoinducer-2 (AI-2)-mediated quorum sensing. In this study, we demonstrated that AI-2-mediated quorum sensing influences the transcription of flaA, the major flagellin gene in Campylobacter jejuni. A null mutation of luxS in C...
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This study was conducted to determine the effects of a high level of infection of the parasitic copepod L. salmonis on the stress response and immunological status of Atlantic salmon. An initial low-level initial infection was carried out 14 d prior to a second infection in which twice as many paras...
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Lepeophtheirus salmonis is an ectoparasitic copepod that can cause serious disease outbreaks in salmonids. Previous work has shown that Atlantic salmon Salmo salar show very little, if any, tissue response to infection, whereas less-susceptible host species exhibit strong inflammatory responses. The...
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Raw river and irrigation water in the Oldman River Basin in southern Alberta was tested for the presence of two bacterial pathogens, Escherichia coli O157:H7 and Salmonella spp., over the last two years (2000-01). The number of E. coli O157:H7 and Salmonella spp. isolated from raw water peaked durin...
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A blastogenesis microassay employing 3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide (MTT) was adapted to measure blastogenic responses of lymphocytes from the chicken's head-associated lymphoid tissues (i.e., the harderian gland and conjunctiva-associated lymphoid tissue) to T- and B-...
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