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The objective of the study was to evaluate the utility of a Petrifilm-based on-farm culture system when used to make selective antimicrobial treatment decisions on low somatic cell count cows (<200,000 cells/mL) at drying off. A total of 729 cows from 16 commercial dairy herds with a low bulk tank s...
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Cow-level milk ELISA results can be used to determine herd Mycobacterium avium ssp. paratuberculosis (MAP) status. Milk sample collection is minimally invasive and ELISA results can be obtained quickly and economically. The objectives were to evaluate the herd-level test characteristics of 3 commerc...
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Mastitis is the main disease entity affecting dairy farms in the Colombian High Plains of northern Antioquia, Colombia. However, no previous epidemiologic studies have determined the characteristics that increase the risk of infection in this region, where manual milking is still the prevailing syst...
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As an alternative to blanket dry cow therapy (BDCT), selective dry cow therapy (SDCT) is considered a more judicious approach to antimicrobial use for the purpose of mastitis control during the non-lactating period. The purpose of this research was to evaluate the utility of a 3M Petrifilm-based on-...
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Simple, rapid and cost-effective methods are sought for measuring immunoglobulin G (IgG) concentrations in bovine serum, which can be applied for diagnosis of failure of transfer of passive immunity (FTPI). The aim of the present study was to investigate the potential use of Fourier-transform infrar...
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Staphylococcus aureus is a significant pathogen frequently causing persistent intramammary infections (IMI) in dairy cows. We compared some genotypic and phenotypic characteristics of 285 strains collected from quarter milk samples from cows with persistent and nonpersistent subclinical IMI across C...
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Mycobacterium avium ssp. paratuberculosis (MAP) is the causative organism of Johne's disease. Although fecal culture is considered the standard diagnostic test, the long incubation times, costs, and intermittent shedding of MAP hinder efficient screening programs based on culture results. The primar...
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Klebsiella spp. is a common cause of bovine mastitis, but information regarding its molecular epidemiology is lacking from many parts of the world. On using mass spectrometry and partial sequencing of the rpoB gene, it was found that over a one year study, K. variicola and Enterobacter cloacae were ...
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This study evaluated test characteristics of environmental culture (EC) for the detection of Mycobacterium avium subspecies paratuberculosis (MAP) in 32 herds over a 2-year period. Individual fecal samples were collected every 6 mo and environmental samples every 3 mo. Individual fecal culture was p...
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The purpose of this study was to evaluate a 3M Petrifilm-based on-farm culture system for the detection of intramammary infection (IMI) in low somatic cell count (SCC) cows (<200,000 cells/mL) at drying off. The main objectives were to determine the test characteristics and the predictive values of ...
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Gastrointestinal nematodes, such as Ostertagia ostertagi and several species of Cooperia, are ubiquitous in temperate climates and have been shown to have detrimental effects on production in adult dairy cattle. A published meta-analysis demonstrated that overall, producers lose approximately 0.35 k...
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The purpose of this research was to evaluate herd and cow-level Mycobacterium avium subspecies paratuberculosis (MAP) diagnostic tests within non-infected and low to moderate within-herd prevalence MAP-infected herds. Specific objectives were to estimate herd-level sensitivity and specificity of env...
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Monitoring of antimicrobial resistance (AMR) in bacteria has clinical and public health significance. The present study determined prevalence of AMR in common mastitis pathogens Staphylococcus aureus, including methicillin-resistant Staph. aureus (MRSA; n=1,810), Escherichia coli (n=394), and Klebsi...
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Major mastitis pathogens such as Staphylococcus aureus, Streptococcus uberis, Streptococcus dysgalactiae, and coliforms are usually considered more virulent and damaging to the udder than minor mastitis pathogens such as Corynebacterium spp. and coagulase-negative staphylococci (CNS). The current li...
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Staphylococcus aureus is the most prevalent pathogen causing intramammary infections (IMI) in dairy cattle worldwide. New treatment regimens have been evaluated to increase cure rates. A systematic review was conducted to answer this question: what is the best antibiotic treatment for S aureus IMI o...
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The primary method of spread for S agalactiae and S aureus is from cow to cow, so prevention focuses on within and between herd biosecurity to reduce or eliminate the reservoir of infection. S agalactiae is an obligate pathogen of the mammary gland, whereas S aureus is more widespread on other cow b...
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An observational study was conducted in 63 Canadian dairy farms to evaluate the association of bulk tank milk selenium (Se) concentration (BTSe) with average daily milk yield, milk fat and milk protein, bulk tank somatic cell count (BTSCC), and the probability of being a Staphylococcus aureus-positi...
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Costs and feasibility of extensive sample collection and processing are major obstacles to mastitis epidemiology research. Studies are often consequentially limited, and fundamental mastitis researchers rarely have the opportunity to conduct their work in epidemiologically valid populations. To miti...
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Bovine herpesvirus-4 (BHV-4), genus Rhadinovirus, is a member of the family Herpesviridae, subfamily Gammaherpesvirinae with a worldwide distribution. This study was conducted to investigate the role of the virus in mastitis and/or subclinical mastitis in the Canadian dairy herds.Milk samples from 1...
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Soils and plants in Atlantic Canadian provinces are known to contain low concentrations of selenium (Se). Earlier studies have indicated that dairy producers in Atlantic Canada are providing insufficient supplementary Se in the ration to meet the Se requirements of dairy cattle, as measured by herd-...
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Our objective was to investigate effects of seropositivity for bovine leukemia virus (BLV), Type 1 bovine viral-diarrhea virus (BVDV), Mycobacterium avium subspecies paratuberculosis (MAP), and Neospora caninum (NC), and their possible interactions, on reproductive efficiency (specifically, first-se...
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Our objective was to determine the risk factors associated with bacteriological quality of bulk tank milk. Bulk tank milk samples were collected from all Prince Edward Island dairy herds (n=235) from March 2005 to March 2007. Biweekly total bacterial, preliminary incubation, laboratory pasteurizatio...
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