In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability...

The total fatty acid composition of rainbow trout (Oncorhynchus mykiss) spermatozoa and seminal plasma was investigated in fresh and in stored semen using gas liquid chromatography. Triglycerides, phospholipids, and cholesterol were measured and the effect of fatty acids, triglycerides, and choleste...

Wichtel. J. J., Charinley. E., Richardson. G. F. Duynisveld. J. L. and Lofstedt. R, 2008. Effects of postpartum energy, intake on pregnancy rates in beef cattle subjected to GnRH- or CIDR-based timed artificial insemination protocols. Can. J. Anim. Sci. 88: 439-447. The objectives were to determine ...

Seminal plasma protein of Arctic char Salvelinus alpinus was. characterized using sodium dodecyl sulfate (SDS) gel electrophoresis. Twelve protein bands with molecular weights of 7.2, 12.4, 15.3, 20.0, 20.4, 22.6, 39.4, 66.3, 74.0, 92.0, 94.5, and 130.1 kilodaltons (kDa) were detected. The effect of...

Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome ...

The objective was to determine the oxidative stability of Arctic char (Salvelinus alpinus) semen following dietary supplementation with lowbush blueberry (Vaccinium angustifolium) product, alpha-tocopherol, alpha-tocopherol+blueberry product, or alpha-tocopherol+astaxanthin. Sperm lipid peroxidation...

The effects of extender composition and freezing rate on motility and fertility of frozen-thawed Arctic char, Salvelinus alpinus, spermatozoa were investigated. Three freezing rates, two semen diluents and three cryoprotectants were tested. Semen frozen in 0.3 mol L-1 glucose diluent with 10% methan...

Scanning electron microscopy (SEM) was used to study the endometrium of nine 1-year-old thoroughbred mares after twice intrauterine infusions of gentamicin, on 2 consecutive days. Five mares were infused on 2 consecutive days with 40 ml gentamicin (50 mg/ml) mixed with 80 ml of normal saline. Four m...

Successful preservation of stallion spermatozoa is difficult because sperm damage occurs from lipid peroxidation during storage. We investigated the use of antioxidants with a standard skim milk extender and several gas environments during storage of stallion spermatozoa to reduce the harmful effect...

The objective of this study was to maintain the viability of chilled rainbow trout (Oncorhynchus mykiss) eyed eggs during storage using oxygenated perfluorochemical (PFC). Three trials were conducted using eggs at 161, 180 or 217 degree days (days from fertilization x incubation temperature in degre...

The effects of dietary lipid from 4 experimental diets on the fatty acid composition and cholesterol content of spermatozoa and their plasma membranes and their consequences for sperm viability after cryopreservation were evaluated in rainbow trout. The 4 sources of lipid were herring oil (adequate ...

Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA...

The possibility of long term storage, by cryopreservation in liquid nitrogen, of spermatozoa of ocean pout (Zoarces americanus) and the changes in motility, fertility and ultrastructure of the spermatozoa after freezing and thawing were examined. Four cryoprotectants, including DMSO, and 3 semen dil...

Although dramatic improvements in growth rates have been documented in growth-enhanced transgenic salmonid fish, prior to commercial implementation of this technology, there is a need for further information relating to the physiology of a number of commercially important production traits. In 1996 ...