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The ontogeny in the pig of effector lymphocytes mediating antibody-dependent and spontaneous cell-mediated cytotoxicity against target cells infected with transmissible gastroenteritis virus was investigated. The same activities were also studied in sows in late pregnancy and early lactation. Periph...
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The purpose of this study was to investigate the ontogeny in the pig of effector lymphocytes mediating antibody-dependent and spontaneous cell-mediated cytotoxicity against target cells infected with transmissible gastroenteritis virus. The same activities were also studied in sows in late pregnancy...
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The objective of this study was to determine whether porcine peripheral blood leukocytes and intestinal intraepithelial leukocytes can mediate antibody-dependent cell-mediated cytotoxicity and spontaneous cell-mediated cytotoxicity against target cells infected with transmissible gastroenteritis vir...
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When porcine peripheral blood leucocytes were fractionated, lymphocytes were the most active effectors in both antibody-dependent cell-mediated cytotoxicity (ADCC) and spontaneous cell-mediated cytotoxicity (SCMC), although both polymorphs and macrophages showed some activity in ADCC. Adsorption of ...
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This review deals with the aetiology and the diagnosis of bovine viral abortion. While the abortion rates on beef and dairy cattle farms usually do not exceed 10%, significant economic losses because of abortion storms may be encountered. Determining the cause of abortions is usually a challenge, an...
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Numerous conventional vaccines for animal use are currently available, and many of these vaccines have been instrumental in the control of infectious diseases of major economic importance. A vaccine has even been instrumental in global eradication of smallpox, an important human disease. However, ma...
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A nested reverse transcriptase PCR (RT-nPCR) was developed for the detection of feline coronavirus (FCoV) RNA in the feces, tissues, and body fluids of infected cats. The RT-nPCR was targeted to the highly conserved 3'-untranslated region of the viral genome and will detect most, if not all, feline ...
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This report identifies and genotypes the bovine viral diarrhoea virus (BVDV) in up to 20 years old archival formalin fixed paraffin embedded (FFPE) tissues of bovine foetuses and calves. Samples from 6 presumably BVDV aborted foetuses and 3 calves diagnosed with mucosal disease were tested by immu- ...
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In two experimental rearing houses with new-born non-colostral calves, separated from their mothers at birth and artificially fed colostrum or colostral whey, the epizootological situation was very favourable throughout the time of observation. The respiratory syndrome occurred in both cases as a re...
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Detection/removal control method of Aleutian disease by CIEP detection of ADV-antibody and farm or barn depopulation/repopulation, have been the recommended approaches of AD control since mid 1970s. The detection/removal was, at least under common N. American husbandry conditions, unsuccessful in co...
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Interaction of native proteinaceous antigens during the recognition and the effector phases of an immune response leads to antigenic conformational modifications which may elicit additional specific immune response. Protein cross-linking and conformation restraining formaldehyde and glutaraldehyde h...
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A significant proportion of infected mink of non-Aleutian genotype naturally resists progression to immune complex disease. The nature of the AD resistance is presumably genetic. While hypergammaglubulinemia can be insensitively detected by an iodine test, more sensitive diagnosis of hyper-γ globul...
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Haemic neoplasia (HN) is a leukemia-like disease that affects at least 20 species of marine bivalves including soft shell clam, Mya arenaria. Since the disease was discovered in 1969, the etiology remains unknown. A retroviral etiology has been suggested based on the detection of reverse transcripta...
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Infectious salmon anemia virus (ISAV) is a very important fish virus in the Northern hemisphere and there is continued interest in understanding the mechanisms of its pathogenesis and persistence in fish. In this study, the permissive fish cell lines SHK-1, CHSE-214 and TO were used to determine whe...
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There is disclosed a process for detecting an RNA virus in a tissue cell or fluid sample, e.g. clinical, suspected of containing the virus. The process comprises heating the sample to a temperature sufficient for protein uncoating the RNA virus, in the presence of an RNase enzyme inhibitor active at...
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The prevalence of bovine viral diarrhea virus (BVDV) persistency was examined in 12 Holstein dairy herds in Charlottetown, Canada, from Feb. 2009 to Oct. 2009. A total of 469 serum and ear notch biopsy pairs were collected. Depending upon the age and vaccination status, the sampled animals were divi...
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Reverse transcriptase (RT) activity has been reported in bivalves affected by haemic neoplasia (HN). Since all retroviruses have RT, detection of RT activity was regarded as evidence for the retroviral etiology of HN. This study investigates the relationship between RT levels and the progress of HN ...