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In this study test characteristics of three newly developed enzyme-linked immunosorbent assays (ELISAs) for Salmonella enterica subsp. enterica serovar Dublin were evaluated and compared with two agglutination tests. The ELISAs involved were an indirect ELISA with serovar Dublin lipopolysaccharide (...
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This study analysed the effects of the use of bovine herpesvirus 1 (BHV1) marker vaccine on the performance of dairy cattle. In Spring of 1999, vaccination of 12 herds with the BHV1 marker vaccine resulted in severe animal health problems and mortality. The vaccines used on these farms were all from...
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In this study, the occurrence of persistent intramammary infections caused by Escherichia coli with recurrent episodes of clinical mastitis caused by E. coli are described for a cohort of 300 Dutch dairy herds. Calculations on the recurrent episodes were based on data collected by dairy farmers. The...
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Four problem herds, totalling 143 cows, with persistent high milk cell counts were investigated. The standard mastitis prevention programme brought about some reduction in cell count, even though its application was incomplete. Bacteriological examination identified 45 cows with Streptococcus agalac...
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A modified procedure was used for culture of Mycobacterium paratuberculosis (Mptb) from bovine feces. Bovine fecal samples were decontaminated with NaOH, exposed to a mixture of oxalic acid and malachite green, incubated in a mixture of neomycin and amphotericin B. Decontaminated specimens were inoc...
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Wildlife, once infected, can serve as a reservoir of infectious diseases that form a constant threat to domestic livestock. To make control and eradication programs successful in the long-term, presence of pestivirus in wildlife populations should be monitored. The goal of this study was to investig...
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Risk factors for the incidence rate of clinical mastitis were studied in 274 Dutch dairy herds. Variables that were associated with resistance to disease were the feeding, housing, and milking machine factors. Variables that were associated with exposure were grazing, combined housing of dry cows an...
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In this study the sensitivity of three methods of isolation of udder pathogens from milk samples from subclinical mastitis cases was compared. For analysis 1827 quarter milk samples were selected. Milk was cultured using a standard culture technique (0.01 ml of fresh milk streaked on a sheep blood a...
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Herd-level sensitivities of bacteriological and serological methods were compared in 79 bovine dairy herds, recently infected with Salmonella enterica subsp. enterica serovar Dublin. All farms experienced clinical signs of salmonellosis for the first time and had no history of vaccination against sa...
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Twelve dairy herds with evidence of post-natal infection with Neospora caninum were compared with 21 control herds with no evidence of post-natal infection. On the former farms, dogs consumed placenta or licked uterine discharge in 75 and 67% of the farms, respectively, while on control farms these ...
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On 6 Dutch dairy farms cows died of an acute, very serious mastitis caused by Pseudomonas aeruginosa. This happened shortly after drying off with antibiotics. Before drying off the teat ends were cleaned with teat wipes contaminated with Pseudomonas aeruginosa.
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On 23 February 1999, the Dutch Animal Health Service advised all Dutch veterinary practices to postpone vaccination against bovine herpesvirus 1 (BHV1) immediately. The day before severe disease problems were diagnosed on four dairy farms after vaccination with the same batch of BHV1 marker vaccine....
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Management style and its association with bulk milk somatic cell count (SCC) and the incidence rate of clinical mastitis were studied in 300 Dutch dairy herds. Cluster analysis was used to identify groups of farmers who had similar management styles for the prevention of mastitis. Two groups of farm...
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To examine the development of teat end callosity thickness and roughness in early lactation and to quantify cow factors of interest, a system to classify teat end condition was developed. A distinction was made between rough and smooth rings around the teat orifice. In addition, a classification of ...
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This study describes an outbreak of bovine herpesvirus 1 (BHV1) infections in a dairy herd with special reference to disease symptoms, reproductive performance and milk production losses. The study was carried out with a dairy herd consisting of 98 lactating animals. All animals were housed in the s...
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Staphylococcus aureus is an important cause of udder infections in dairy herds. Both lactational and dry cow therapy are part of Staph. aureus control programs. Reported cure rates for Staph. aureus mastitis vary considerably. The probability of cure depends on cow, pathogen, and treatment factors. ...
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Clinical mastitis (CM) is one of the major diseases in dairy herds. It induces economic costs, mainly consisting of discarded milk, increased health care costs and reduced milk quality. Mastitis also contributes to consumer concerns regarding animal welfare and regarding the impact of use of antibio...
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In the Netherlands a survey was conducted to estimate the prevalence of paratuberculosis in dairy herds. In total 15822 cows of at least 3 years of age, belonging to 378 herds were tested using an absorbed ELISA. Of these herds, 55% (n=207) had one or more serologically positive cows. Of the positiv...
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An outbreak of Streptococcus uberis mastitis was described to gain insight into the dynamics of Strep. uberis infections at a herd level. Data were obtained from a longitudinal observational study on a commercial Dutch dairy farm with good udder health management. Quarter milk samples for bacteriolo...
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Paratuberculosis in cattle is a chronic debilitating infectious disease caused by Mycobacterium paratuberculosis. Control of paratuberculosis is based on tests that principally detect advanced stages of infections: faecal culture and serology. Tests measuring cell-mediated immunity (CMI) could impro...
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Earlier field observations suggest that teat apex colonization by Staphylococcus chromogenes pre-partum in dairy heifers protects udder quarters against elevated somatic cell counts early post-partum. To explain these findings, the in vitro inhibitory capability of S. chromogenes from teat apices of...
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Genetic associations were estimated between pathogen-specific cases of clinical mastitis (CM), lactational average somatic cell score (LACSCS), and patterns of peaks in somatic cell count (SCC) which were based on deviations from the typical lactation curve for SCC. The dataset contained test-day re...
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Results are described of a split-udder trial on the effect of discontinuation of postmilking teat disinfection on the incidence of clinical mastitis in seven dairy herds with a low bulk milk somatic cell count and a high incidence of clinical mastitis. Overall incidence of clinical mastitis was non-...
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The herd health approach for dairy herds with a high bulk milk somatic cell count, in which Streptococcus agalactiae plays a major role, was evaluated. After introduction of the standard mastitis prevention programme, all quarters of infected cows were treated during lactation. In three of the four ...