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Sporozoites of S. hirsuta, although they also penetrated into bovine monocytes, developed to the schizogonous phase only in bovine pulmonary artery endothelial cells. Schizonts were evident from 14 days after sporozoite inoculation (DAI) and persisted to 62 DAI, when experiments were terminated. Mer...
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Spores identified as Myxobolus neurobius were found in head homogenates of both wild young Atlantic salmon Salmo salar and Arctic char Salvelinus alpinus . Formalin-preserved spores from Atlantic salmon measured an average of 12.9 x 9.4 mu m and contained two polar capsules that measured 6.4 x 2.9 m...
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The levels of production, ascarid burden and respiratory disease were measured on 15 purposively selected swine herds, and the relationships between the various measures of ascarid burden were examined. On each farm 30 randomly selected pigs were weighed and rectal fecal samples were collected at ap...
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Proteinase K was used for the cleaning of Sarcocystis cruzi (Apicomplexa) sporocysts prior to excystation. Bovine pulmonary endothelial cell cultures inoculated with the excysted sporozoites remained free of bacterial contamination for the duration of the experiment and had high yields of merozoites...
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The principal lesions due to the ciliate Anophryoides haemophila in experimentally infected lobsters are observed in the gills and connective tissue from the 9th week post-infection. These lesions were not related to the inoculum of parasites and were consistent in all inoculum studies (2000, 10 000...
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The concentration of arginine kinase (AK) in the hemolymph of lobsters may aid in the identification of muscle diseases in these animals in the same way that creatine kinase activity in blood plasma does in mammalian species. Both manual and automated analysis methods were developed for measuring AK...
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Mushroom tyrosinase (E.C. 1.14.18.1) was evaluated as a control material for use in a plasma prophenoloxidase assay in the American lobster Homarus americanus as part of the assay's validation procedure. Reconstituted. lyophilized tyrosinase solution was stable for 72 h at 2-4degreesC. Percent ...
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American lobsters Homarus americanus were inoculated with a field isolate of the Gram-positive bacterium Aerococcus viridans var. homari, causative agent of gaffkemia, at 1 x 10(6), 1 x 10(4) or 1 x 10(2) colony forming units (CFU) kg(-1) or with sterile 3% NaCl and maintained at 10 or 15 degrees C ...
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The purpose of this research was to investigate the salinity and formalin sensitivity of a ciliate parasite (Anophryoides haemophila) of the American lobster (Homarus americanus), and to examine the target-animal (lobster) safety of chemical-bath treatments involving low salinity, formalin, or chlor...
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Reference ranges for blood chemical and cellular components in the "healthy" population of interest are required to identify alterations in individuals in association with disease and disorders. The purpose of this study was to determine reference ranges for various constituents of hemolym...
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A combination of 16S rRNA sequencing and random amplified polymorphic DNA (RAPD) analysis was used to evaluate the genetic diversity within Aerococcus viridans var. homari, the causative agent of gaffkemia in lobsters. A collection of 7 A. viridans var. homari strains and 2 avirulent A. viridans-lik...
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Chronic mortalities in lumpfish Cyclopterus lumpus reared in a saltwater recirculation system were associated with the presence of an intranuclear microsporidian. Morphological changes were characterized by infiltration of lymphocyte-like cells predominantly in the renal interstitium and also in the...
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Potassium chloride (KCl: 330 mg/ml) was assessed as an euthanasia agent in American lobsters (Homarus americanus). Two groups of 10 lobsters (408.2 to 849.9 g) were maintained at 11.9 to 12.1 degrees C ('warm') and 1.5 to 2.5 degrees C ('cold') to evaluate the possible effect of ...
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The pathology caused by the ciliate Anophryoides haemophila was studied in experimentally infected lobsters. The first lesions due to the parasite were observed in gills and connective tissue from 9th week post-infection for the lower inocula and at week 4 for the 500,000 ciliate inoculum.. These le...
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Adult ticks were found on 2 cats and a dog in the Charlottetown area between June and November 1989. The 3 ticks were identified as I. dammini. Serum samples from the 2 cats were examined for antibodies to Borrelia burgdorferi; IFA titres were less than 1:32 in both cats. It is uncertain whether in ...
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This paper presents an overview of perkinsiosis in Salmo salar farmed in the Bay of Fundy, Canada, including information on the systematics of Perkinsus parasites, infection and disease caused by Perkinsus, lesions associated with the parasite in smolt and growout (market weight) salmon, differentia...
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Neoparamoeba pemaquidensis continues to be an ongoing problem for commercial finfish aquaculture and has also sporadically been associated with mass mortalities of commercially relevant marine invertebrates. Despite the ubiquity and importance of this amphizoic amoeba, our understanding of the biolo...
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We have performed a genomic characterization of a kinetoplastid protist living within the amoebozoan Neoparamoeba pemaquidensis. The genome of this "Ichthyobodo-related organism" was found to be unexpectedly large, with at least 11 chromosomes between 1.0 and 3.5 Mbp and a total genome size of at le...
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Hematodinium is a parasitic dinoflagellate of numerous crustacean species, including the economically important Atlantic snow crab, Chionoecetes opilio. The parasite was isolated from C. opilio in the fall of 2010 and 2011 and cultured in vitro. Several media were tested, with Nephrops medium yieldi...
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Trichocyst morphology and development were explored using transmission electron microscopy in Hematodinium spp. isolated directly from Atlantic snow crab (Chionoecetes opilio) hemolymph and from in vitro cultures. Appearance of trichocysts defines the initiation of a morphological transition in the ...
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The objective of this study was to identify risk factors for productivity losses in the Canadian lobster industry. Lobster handling and fishing practices onboard 64 fishing boats and transportation conditions on 49 vehicles subsequently transporting these lobsters from fishing wharves to processing ...