A 2-year old gelding was referred for evaluation of severe right forelimb lameness. The horse was grade 4/5 lame on the right forelimb. Clinical, laboratory, and radiographic findings were consistent with septic arthritis and osteomyelitis. Due to poor prognosis the owner elected euthanasia. Histopa...

Background Recent focus on earlier detection of pathogen introduction in human and animal populations has led to the development of surveillance systems based on automated monitoring of health data. Real- or near real-time monitoring of pre-diagnostic data requires automated classification of recor...

Cloacal and pharyngeal swabs from 100 tree-nesting Double-crested cormorant (DCC) chicks were examined by culture for commensal and potentially pathogenic bacteria. No Salmonella or Erysipelothrix were isolated from the cloacal swabs. Twenty-two cloacal swabs were positive for Campylobacter, of whic...

In the summer of 1999, the incidence of Salmonella enterica serotype infantis infections in Alberta rose dramatically. Subsequent laboratory and epidemiological investigations established that an outbreak of human disease caused by this organism was occurring across Canada and was associated with pe...

Canadian Food Inspection Agency (CFIA) has adopted the term cyanosis to describe a category of condemnation for poultry that is dark but has no other condemnable lesions. Two case-control studies (n = 30 pairs; n = 65 pairs) of 18-wk-old tom turkeys were conducted. A case was defined as a carcass co...

Antibodies to seven antigens in a whole cell lysate of Corynebacterium pseudotuberculosis ranging in molecular mass from 22 to 120 kilodaltons (kDa) were present in sera of 40 sheep and goats infected with C. pseudotuberculosis. Three antigens of about 120, 68, and 31.5 kDa in size were consistently...

An antibody microarray assay was developed for Salmonella serotyping based on the Kauffmann-White scheme. A model (8 by 15) array was constructed using 35 antibodies for identification of 20 common Salmonella serovars and evaluated using 117 target and 73 nontarget Salmonella strains. The assay allo...

In vitro methods were used to test the hypothesis that Escherichia coli from bovine mastitis are essentially no different from isolates from bovine feces. Fifty E. coli isolates from bovine mastitic milk, 50 from feces of mastitic cows and 50 from feces of healthy cows were compared with respect to ...

In order to describe the farm-level Salmonella status, 113 Ontario swine farms were tested for Salmonella one to five times within the time period 2001-2006. During 422 visits, 6844 fecal samples were collected and cultured for Salmonella. Salmonella was recovered from 437 (6.38%) of the fecal sampl...

Raw and pasteurized milk samples submitted for routine quality analysis were screened for the presence of B. cereus diarrhoeal enterotoxin (BDE) using the TECRA BDE visual immunoassay (VIA) kit. BDE was not detected in 298 raw milk samples tested by the TECRA VIA. B. cereus was isolated from 2 of 29...

A clinical guide to the care of domestic, laboratory, and zoo animals that contains information on nutrition, physiology, medical disorders, and methods of treatment.

The Sceptor system (Becton Dickinson Diagnostic Instrument Systems, Towson Md.) was assessed for its ability to identify veterinary clinical isolates. A total of 605 bacteria, including 315 isolates of the family Enterobacteriaceae, 191 gram-negative nonenteric bacteria, and 99 gram-positive bacteri...

The present study was undertaken to estimate the occurrence and distribution of multidrug resistance (MDR) among Salmonella serovars isolated from slaughtered pigs at Debre Zeit, Ethiopia. A total of 501 different samples were examined of which 42 (41.6%) of 101 mesenteric lymph nodes, 22 (21.8%) of...

The aim of this study was to determine the farm prevalence of Salmonella in 90 Alberta finishing swine farms over a 5-month period, to evaluate Salmonella distribution in the farm environment and to describe Salmonella serovar diversity on these farms. Ten veterinary practitioners selected 90 Albert...

Twenty-five isolates of Corynebacterium pseudotuberculosis from lesions of caseous lymphadenitis in goats were examined for cell wall lipid content, exotoxin production, and virulence in mice. The mean percentage of lipid content was 6.52% and significant (P less than 0.005) differences were demonst...

Specificity has been a problem with tests for C. pseudotuberculosis, probably due to cross-reaction with non-specific antigens that are contained in crude preparations of antigen. An ELISA has been developed for the detection of antibodies to the phospholipase D (PLD) exotoxin of C. pseudotuberculos...

14 early lactation Holstein cows were experimentally challenged by intramammary infusion of 2 teats to compare the virulence of the strains of Escherichia coli isolated from faeces or from cases of bovine mastitis. The initial treatment protocol consisted of parenteral antibiotics and fluid therapy ...

The Sceptor System (Becton Dickinson) was compared with an agar dilution method for antimicrobial susceptibility testing of veterinary clinical isolates. The results indicate that the Sceptor System may be used to test gram-positive and fastidious gram-negative bacteria.