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The goal of the present study was to compare the effect of temperature on contraction kinetic parameters of fish muscle with its effect on oscillatory work. In particular we studied the effect of stimulus train duration or duty cycle (the fraction of the imposed length change that the muscle was sti...
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In this study, we injected morphine sulfate IP into rainbow trout and measured the concentration of morphine and all potential metabolites in plasma using LC-MS/MS at a series of times after the injection. The pharmacokinetics of morphine were similar to those previously reported for seawater-acclim...
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The major fraction of elevated oxygen uptake of liver and skeletal muscle tissue slices that occurs during cold adaptation in mice is ouabain sensitive.
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Acoustic telemetry showed that stomach temperature from large bluefin tuna, Thunnus thynnus, held in an impoundment, changed during feeding. The stomach cooled rapidly on ingesting cold food. It then warmed to a maximum of 10° to 15°C above water temperature over a period of 12 to 20 h. Temperatur...
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Isometric twitch and tetanus parameters, force-velocity curves, maximum shortening velocity (V-max) and percentage relaxation between stimuli (%R) across a range of stimulus frequencies were determined for a muscle used during call production (the tenser chordarum) and a locomotor muscle (the sartor...
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Rainbow trout were sprint-trained (30 s duration) once or twice on alternate days for 6 weeks. Swim speed for the first 10 s of a training bout averaged 11.4 body lengths per second (bl/s) for group 2 (trained once) and 10.2 bl/s for group 3 (trained twice). Feed intake, growth rate and conversion e...
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1. 1.|Decreasing temperature stimuli produced responses and threshold similar to those produced by increasing temperature stimuli in a previous study with skipjack tuna, Katsuwonus pelamis. 2. 2.|Restrained skipjack tuna signaled by deceleration of heart rate that they could perceive abrupt temperat...
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We investigated the influence of oxygen demand (developmental stage) and supply (hypoxia, water flow rate, the chorion and body movements) on the oxygen concentration within the boundary layer next to the chorion of embryos or skin of larvae of rainbow trout (Oncorhynchus mykiss). Oxygen microelectr...
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A popular part of many athletes pre-game regime is to stretch. We examined whether a pre-injury stretching protocol could prevent acute contraction-induced injury. The in situ extensor digitorum longus (EDL) muscle of an anesthetized mouse (80 mg/kg intra-peritoneal) was used. Damage to the muscle f...
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Most athletes stretch as part of their training regimen and it is commonly believed that this practice prevents muscle injury. We tested this belief using an animal model, in situ mouse extensor digitorum longus (EDL) muscle. One lower hindlimb was slowly stretched for 1 min on alternate days for 12...
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The goal of our experiment was to elucidate the effect of stimulus duty cycle (the percentage of the cycle that the muscle was stimulated), phase (the relative timing of the imposed sinusoidal length change and stimulation), and muscle cycle frequency (the speed at which the muscle was cycled) on wo...
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To test predictions of biochemical symmorphosis, we measured the activity of seven consecutive glycolytic enzymes at three positions along the heterothermic white muscle of the bluefin tuna. Biochemical symmorphosis predicts that adjustments in sequential enzyme concentrations along a thermal gradie...
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We measured enzyme activities along a heterothermic tissue, the visceral retia mirabilia of the bluefin tuna, to test current theories of enzyme temperature adaptation. The heterothermic tissue model is ideal for the study of fundamental temperature adaptation because it eliminates confounding effec...
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Swimbladder walls of lake charr, Salvelinus namaycush, from Great Slave Lake (GSL), Northwest Territories, Canada, were unusually thick for the species. The thinnest sections of the GSL bladders (mean = 2.44mm, range = 1.1-4.4mm) were significantly thicker (P = 0.001) than lake charr swimbladders co...
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We examined the thermal biology of free-ranging terrestrial eastern foxsnakes (Elaphe gloydi Conant, 1940) that were voluntarily swimming in cold water during spring, in Georgian Bay, Ontario, Canada. Using temperature-sensitive radiotelemetry, we recorded body temperatures of foxsnakes during 12 co...