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Effective methods of traceability are urgently required for use in research as well as in different types of aquaculture operations and to control trade in aquatic animals and products. In regard to the marking of fish, many different tagging methods have been described and the method to be used dep...
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The protozoan parasite Perkinsus atlanticus (Azevedo, 1989) causes severe losses among cultured clams, Ruditapes decussatus. This parasite is routinely diagnosed by means of histology or incubation of gills in fluid thioglycollate medium. However, in order to develop models of experimental reproduct...
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The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains ...
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A nested polymerase chain reaction (PCR) has been developed for the diagnosis of Marteilia refringens in mussels (Mytilus galloprovincialis) from Galicia (NW Spain). Specific primers were used in two consecutive amplification reactions, that were able to detect 15 fg of Marteilia DNA. The nested PCR...
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The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains ...
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In 1999, the world aquaculture production of molluscs was estimated at 9.4 million tonnes which represents approximately a 23% contribution to the global aquaculture production. Aquaculture of molluscs has been growing at an average annual rate of 11% over the past decades, although some activities ...
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Disseminated neoplasia (DN) is a disorder referred to as hemic neoplasia (HN) in the soft-shell clam Mya arenaria. Traditionally, diagnosis is performed by hematocytology or histology. The intensity of the disease is generally given as the percentage of transformed neoplastic cells Out of total numb...
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A parasite of the dredge oyster Ostrea chilensis Philippi, previously known as Bonamia sp., was recently described and named Bonamia exitiosus, based on ultrastructural and molecular evidence. The rules governing the names of organisms require correspondence of gender, and the name must be changed f...
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The flat oyster Ostrea chilensis is susceptible to infection with two microcells, Bonamia exitiosa and Bonamia ostreae. A haemocytic parasite very similar to Bonamia species has been previously reported in two of 167 Chilean oysters sampled from Chiloe Island in 1996-1997. In order to characterise t...
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The molecular mechanisms by which haemocytes of clams are transformed in the course of haemic neoplasia remain by far unknown. The aim of this study was to quantify the expression of p53/p73 and mortalin genes, in relation with the ploidy status of clam haemocytes and to correlate the p53 expression...
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The life history of Marteilia refringens was investigated. The infectivity of purified early and late stages of M. refringens to oysters (Ostrea edulis) was tested in the laboratory using different routes of inoculation. Naturally infected oysters were monitored in experimental tanks to study infect...
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This supplement presents the results of a survey on Mediterranean aquaculture diagnostic laboratories, which has been implemented by the Mediterranean Institute of Zaragoza (IAMZ) in collaboration with the FAO Fisheries Department. The aim of the survey is to acquire greater knowledge on the Mediter...
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Bonamia ostreae is a protozoan parasite of the flat oyster, Ostrea edulis, which has caused significant loss of oysters in Europe over the last decade. B. ostreae was purified from infected flat oysters and DNA was extracted. The nearly complete small subunit rDNA gene of B. ostreae was amplified us...
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In France abnormal mortalities of mollusks affect many species of bivalves. They occur mainly in summer and concern all the French coastline. For Crassostrea gigas, they affect all life's stages but more particularly spat. A pathology monitoring network, REPAMO, was created at the beginning of ...
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The pathogens related to summer mortality outbreaks are a herpes virus and two bacterial strains one belonging to Vibrio splendidus biovar II and the other to Vibrio splendidus spp. However, the feature pathogen/opportunist of these strains is still unknown. Several strains belonging to the genus Vi...
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Marteilia refringens is recognized as one of the most significant pathogens of bivalve molluscs. The nucleotide sequence of the small subunit ribosomal RNA gene of Marteilia refringens is used to elucidate the phylogenetic position of the phylum Paramyxea. Genomic DNA was extracted from sporangia of...
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Original Abstract: L'elevage des huitres repose sur des transferts de coquillages - et donc d'agents pathogenes - entre pays, voire entre continents. Des methodes d'identification pemettent aujourd'hui de retracer cette histoire compliquee, et laissent entrevoir une meilleure protection du cheptel f...
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