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Original Abstract: Le protozoaire parasite Marteilia refringens est responsable d'importantes mortalites au sein des elevages d'huitres plates Ostrea edulis, et a participe a la disparition de 90% de la production depuis les annees 60. Une gestion optimale des risques de contamination des huitres pl...
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Diagnosis of haemic neoplasia (HN) in the soft shell clam, Mya arenaria, is often achieved by hematocytology and histology. Since neoplastic cells display tetraploid DNA contents, haemocyte cell cycle analysis was developed for use as a diagnosis tool. The aim of this study was to assess the applica...
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Gene expression Studies have opened a tremendous field of investigation in biological research over the last decades. Expression of genes is most frequently quantified by real time PCR (RT-qPCR), as this method has proven to be highly sensitive. One of the critical steps, however, in comparing trans...
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Effective methods of traceability are urgently required for use in research as well as in different types of aquaculture operations and to control trade in aquatic animals and products. In regard to the marking of fish, many different tagging methods have been described and the method to be used dep...
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Since its first description, the paramyxean parasite Marteilia refringens has been recognized as a significant pathogen of bivalve mollusks. The existence of a complex life cycle was postulated by many authors. Here we report the development of DNA-based detection assays as powerful tools to elucida...
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The protozoan parasite Perkinsus atlanticus (Azevedo, 1989) causes severe losses among cultured clams, Ruditapes decussatus. This parasite is routinely diagnosed by means of histology or incubation of gills in fluid thioglycollate medium. However, in order to develop models of experimental reproduct...
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The development of molecular diagnostic assays with increased sensitivity compared with conventional histological techniques is highly desirable for effective management of bonamiosis in cultured oyster stocks and wild populations. A real-time TaqMan PCR assay was developed for the specific detectio...
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The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains ...
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One of limiting factors in shrimp culture is the mortality at the larval stage. Often bacterial diseases are suspected to be the origin of this damage. This work concerns the study of relations between microorganisms (bacteria and Vibrio) and peneids. Observations induced a new approach to bacteriol...
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Microcell-type parasites of oysters are associated with a complex of diseases in different oyster species around the world. The etiological agents are protists of very small size that are very difficult to characterize taxonomically. Associated lesions may vary according to the host species, and the...
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A nested polymerase chain reaction (PCR) has been developed for the diagnosis of Marteilia refringens in mussels (Mytilus galloprovincialis) from Galicia (NW Spain). Specific primers were used in two consecutive amplification reactions, that were able to detect 15 fg of Marteilia DNA. The nested PCR...
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The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains ...
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Primers and DNA probes designed for use in the specific detection of the paramyxean parasites Marteilia sydneyi and Marteilia refringens were tested for their potential to cross-react with closely related species in Polymerase Chain Reaction (PCR) and in situ hybridization. PCR primers and a DNA pro...
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Since 1993, significant mortality outbreaks of Penaeus stylirostris usually during winter, have been reported from grow out ponds in New Caledonia. During the mortality peaks in 1994 and 1995, moribund and apparently healthy shrimps were collected from all New Caledonian farms for bacteriological an...
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In 1999, the world aquaculture production of molluscs was estimated at 9.4 million tonnes which represents approximately a 23% contribution to the global aquaculture production. Aquaculture of molluscs has been growing at an average annual rate of 11% over the past decades, although some activities ...
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In 2004, epizootiological studies were conducted on mass mortalities of tunicates Halocynthia roretzi in Goje, Korea. The clinical characteristics of infected H. roretzi were weakness of the tunic, loss of elasticity, and finally death involving a rupture of the tunic. Histological studies revealed ...
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An ultrastructural study was carried out on Mikrocytos mackini, the cause of Denman Island disease in Pacific oysters Crassostrea gigas in western Canada. Three forms were identified, quiescent cells (QC), vesicular cells (VC) and endosomal cells (EC). QC occurred in the vesicular connective tissue ...
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In Argentina, especially in San Matias Gulf, oyster culture is a recent activity. Culture of native flat oyster, Ostrea puelchana (d'Orbigny, 1842), was particularly promising until an abnormal mortality occurred in 1996. Preliminary diagnosis, based on histology, revealed the presence of Bonamia-li...
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Disseminated neoplasia (DN) is a disorder referred to as hemic neoplasia (HN) in the soft-shell clam Mya arenaria. Traditionally, diagnosis is performed by hematocytology or histology. The intensity of the disease is generally given as the percentage of transformed neoplastic cells Out of total numb...
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A parasite of the dredge oyster Ostrea chilensis Philippi, previously known as Bonamia sp., was recently described and named Bonamia exitiosus, based on ultrastructural and molecular evidence. The rules governing the names of organisms require correspondence of gender, and the name must be changed f...
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Marteiliosis is a disease of molluscs caused by Marteilia refringens in Europe and M. sydneyi in Australia. During routine examination of cultured mussels Mytilus galloprovinciallis in the northern Adriatic, the occurrence of Marteilia sp. was recorded with a prevalence of 5%. This parasite was not ...
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The flat oyster Ostrea chilensis is susceptible to infection with two microcells, Bonamia exitiosa and Bonamia ostreae. A haemocytic parasite very similar to Bonamia species has been previously reported in two of 167 Chilean oysters sampled from Chiloe Island in 1996-1997. In order to characterise t...