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Gastrointestinal nematodes rarely cause signs of clinical disease in adult cattle. However, they have been shown to exert a negative impact on production in lactating animals, as seen by improved production following elimination of the worms using anthelmintics. A double blind, randomized clinical t...
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The epidemiology of bovine gastrointestinal nematodes was investigated through a 1-year (October 1999 to September 2000) longitudinal study in 38 Canadian dairy herds from 4 different provinces (Prince Edward Island, Quebec, Ontario, Saskatchewan). For each herd, fecal egg counts from 8 randomly sel...
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We evaluated (using latent-class models) the ability of the California Mastitis Test (CMT) to identify cows with intramammary infections on the day of dry-off. The positive and negative predictive values of this test to identify cows requiring dry-cow antibiotics (i.e. infected) was also assessed. W...
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The purpose of this research was to determine the effect of seropositivity for exposure to bovine leukaemia virus (BLV), Mycobacterium avium subspecies paratuberculosis (MAP) and Neospora caninum (NC) on calving to conception interval (CCI) in dairy cattle in three eastern Canadian provinces. Sample...
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Our objective was to determine the risk factors associated with bacteriological quality of bulk tank milk. Bulk tank milk samples were collected from all Prince Edward Island dairy herds (n=235) from March 2005 to March 2007. Biweekly total bacterial, preliminary incubation, laboratory pasteurizatio...
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The present study was carried out to evaluate the relationship between milk optical density ratios (ODRs) from an indirect Ostertagia ostertagi ELISA, total milk IgG levels and milk production and then establish a correction factor to adjust ODR. Five hundred and sixty composite milk samples collect...
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The purpose of this research was to determine the effects of seropositivity for exposure to bovine leukemia virus (BLV), bovine viral diarrhoea virus (BVDV), Mycobacterium avium subspecies paratuberculosis (MAP) and Neospora caninum (NC) on overall and reason-specific culling in Canadian dairy cattl...
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An observational study was conducted in 63 Canadian dairy farms to evaluate the association of bulk tank milk selenium (Se) concentration (BTSe) with average daily milk yield, milk fat and milk protein, bulk tank somatic cell count (BTSCC), and the probability of being a Staphylococcus aureus-positi...
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Enzyme-linked immunosorbent assays (ELISAs) are used in Johne's disease (JD) control programs as a first screening for presence of the disease in a herd. A high sensitivity of the ELISA is therefore important, yet the commonly used ELISAs have relatively low sensitivity. The inclusion of an abs...
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Compared with blanket dry cow therapy (DCT), the selective antimicrobial treatment of cows based upon on-farm culture results has the potential to reduce the amount of antimicrobials used in dairy production. The objective of the current study was to determine the effect of a Petrifilm (3M Canada, L...
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Gastrointestinal nematodes, such as Ostertagia ostertagi and several species of Cooperia, are ubiquitous in temperate climates and have been shown to have detrimental effects on production in adult dairy cattle. A published meta-analysis demonstrated that overall, producers lose approximately 0.35 k...
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The objective of this study was to assess the stability of ELISA plates prepared with one of three blocking agents and used with one of two conjugates at various time intervals after preparation of the plates. Two of the blocking agents used were commercially available: one termed stabilgaurd (stab)...
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Paired samples of formalin-fixed, paraffin-embedded ileum and lymph node from 204 culled dairy cows were investigated for evidence of infection by Mycobacterium avium subsp. paratuberculosis. Of the samples, 151 were from animals that were tissue-culture positive for M. avium subsp. paratuberculosis...
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The prevalence of Mycobacterium avium ssp. paratuberculosis (Mptb) in culled dairy cattle in Eastern Canada and Maine was determined to be 16.1% (95% confidence interval 13.8 to 18.3%) based on a systematic random sample of abattoir cattle. Mesenteric lymph nodes and ileum from 984 cows were examine...
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