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The general objective of this study was to evaluate a crude Ostertagia ostertagi antigen enzyme-linked immunosorbent assay (ELISA) for monitoring gastrointestinal parasites in lactating dairy cattle. A longitudinal study of gastrointestinal parasites in lactating dairy cows was carried out in 38 her...
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Survey responses from 75 randomly selected dairy producers on Prince Edward Island were summarized to obtain a demographic picture of the dairy industry in this province and to determine information management practices and demands for the future. The results indicate a preparedness for dairy produc...
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During a 10-month period in 1999, 994 serum and tissue samples were collected from dairy cows at slaughter in eastern Canada. The sources of these cattle were from all four Atlantic Canadian provinces along with some cows from the state of Maine. The sera were used to assess the agreement of three c...
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Monitoring of antimicrobial resistance (AMR) in bacteria has clinical and public health significance. The present study determined prevalence of AMR in common mastitis pathogens Staphylococcus aureus, including methicillin-resistant Staph. aureus (MRSA; n=1,810), Escherichia coli (n=394), and Klebsi...
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OBJECTIVE: To determine whether half-udder intramammary infusion of cloxacillin results in transfer of cloxacillin from treated to untreated mammary gland quarters within nonlactating cows, and, if so, at what concentrations, and to determine whether selection of ipsilateral versus diagonal-contrala...
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Our objective was to describe cow and quarter-level factors associated with drying-off, and to evaluate their impacts on new intramammary infections (IMI) during the dry period. Data from 300 cows in five research herds were collected starting 2 weeks prior to scheduled drying-off. Variables of inte...
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Our objective was to investigate effects of seropositivity for bovine leukemia virus (BLV), Type 1 bovine viral-diarrhea virus (BVDV), Mycobacterium avium subspecies paratuberculosis (MAP), and Neospora caninum (NC), and their possible interactions, on reproductive efficiency (specifically, first-se...
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Milk samples from 50 sheep on a single Scottish research farm were collected weekly for 10 wk postpartum. Samples were analyzed for somatic cell counts (SCC) each week and bacteriologic culture was done for 7 of the 10 wk. A total of 492 udder half samples were cultured, of which 467 had correspondi...
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An observational study was conducted in 63 Canadian dairy farms to evaluate the association of bulk tank milk selenium (Se) concentration (BTSe) with average daily milk yield, milk fat and milk protein, bulk tank somatic cell count (BTSCC), and the probability of being a Staphylococcus aureus-positi...
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The objectives of this study were: 1) to determine if bulk tank milk urea nitrogen (BTMUN) and whole herd weighted average of the individual cow MUN levels (WHMUN) were equivalent measurements of herd MUN status; and 2) to determine the seasonal variation in BTMUN concentrations in Prince Edward Isl...
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A clinical trial was conducted to determine the perceived and actual utility of milk urea nitrogen (MUN) notification and interpretation as a tool for monitoring protein and energy imbalances in dairy cows. Based on MUN results during the summer of 2001, 50 farms with high MUN values and 30 with low...
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Although there is increasing concern over routine administration of long-acting antibiotics to all cows at the end of lactation, the practice is required to eliminate subclinical infections that are present at drying off. Alternatives to dry cow therapy (DCT) for preventing new infections from occur...
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Paired samples of formalin-fixed, paraffin-embedded ileum and lymph node from 204 culled dairy cows were investigated for evidence of infection by Mycobacterium avium subsp. paratuberculosis. Of the samples, 151 were from animals that were tissue-culture positive for M. avium subsp. paratuberculosis...
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Enzyme-linked immunosorbent assays (ELISAs) are used in Johne's disease (JD) control programs as a first screening for presence of the disease in a herd. A high sensitivity of the ELISA is therefore important, yet the commonly used ELISAs have relatively low sensitivity. The inclusion of an abs...
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The prevalence of Mycobacterium avium ssp. paratuberculosis (Mptb) in culled dairy cattle in Eastern Canada and Maine was determined to be 16.1% (95% confidence interval 13.8 to 18.3%) based on a systematic random sample of abattoir cattle. Mesenteric lymph nodes and ileum from 984 cows were examine...
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