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Five bulls with high levels of sperm-cell defects were evaluated for breeding soundness approx. once a month for several months. Considerable variation with time was observed in the levels of normal and abnormal sperm cells. These variations reflected only minimally on the breeding soundness ratings...
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The accuracy of three in vitro methods for estimating the proportion of dead rainbow trout (Onchorhynchus mykiss ) spermatozoa was investigated. Motility rating, fluorometry using ethidium bromide, and lactate dehydrogenase (LDH) activity in seminal plasma were compared. Semen samples were prepared ...
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The effects of extender composition and freezing rate on motility and fertility of frozen-thawed Arctic char, Salvelinus alpinus, spermatozoa were investigated. Three freezing rates, two semen diluents and three cryoprotectants were tested. Semen frozen in 0.3 mol L-1 glucose diluent with 10% methan...
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Successful preservation of stallion spermatozoa is difficult because sperm damage occurs from lipid peroxidation during storage. We investigated the use of antioxidants with a standard skim milk extender and several gas environments during storage of stallion spermatozoa to reduce the harmful effect...
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Although dramatic improvements in growth rates have been documented in growth-enhanced transgenic salmonid fish, prior to commercial implementation of this technology, there is a need for further information relating to the physiology of a number of commercially important production traits. In 1996 ...
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In two trials, Arctic char (Salvelinus alpinus) semen was frozen in 0.5 mL straws using extenders consisting of 0.3 M glucose and 10%, 12.5% or 15% methanol. Cryopreserved semen was thawed by immersing straws in 25°C water for 17 s (11.6°C s-1) or in 5°C water for 60 s (3.3°C s-1). The viability...
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This study investigated the relationship between fertility and fatty acid (FA) profiles of egg chorions, total egg, and sperm cells of Arctic char, Salvelinus alpinus (L.) broodstock housed in tanks and fed a commercial diet. Fertility of semen from individual males was assessed using pooled eggs, a...
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Egg quality in Arctic char, Salvelinus alpinus, kept indoors at 7 °C during spawning season was morphologically classified. Four egg categories based on lipid droplet distributions and egg diameter were characterized. Eggs with homogenous lipid vesicle distributions and a uniform size were classif...
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Semen from yellowtail flounder was cryopreserved in 0.25 ml and flat 1.7 ml straws. Semen frozen in 1.7 ml straws was collected from males that had been stimulated to increase semen output by treatment with a GnRH analogue. The semen extender was a modification of a diluent that had previously been ...