Semen from yellowtail flounder was cryopreserved in 0.25 ml and flat 1.7 ml straws. Semen frozen in 1.7 ml straws was collected from males that had been stimulated to increase semen output by treatment with a GnRH analogue. The semen extender was a modification of a diluent that had previously been ...

Lower fertility of preserved stallion semen may be caused by damage to the spermatozoa or premature capacitation during storage. We investigated the use of the flavonoid, quercetin, with a standard skim milk extender for storage of stallion spermatozoa to prevent premature capacitation and acrosome ...

The effects of dietary lipid from 4 experimental diets on the fatty acid composition and cholesterol content of spermatozoa and their plasma membranes and their consequences for sperm viability after cryopreservation were evaluated in rainbow trout. The 4 sources of lipid were herring oil (adequate ...

Cryopreservation of Arctic charr semen was investigated using 3 diluents (0.3 M glucose, 0.3 M glucose with 0.011 M KCl and 0.137 M NaCl, 0.011 M KCl, 0.004 M Na2HPO4 with 7.5 g/litre L- alpha -lecithin adjusted to pH 7.5) 3 cryoprotectants [10% dimethyl sulphoxide (DMSO), 10% dimethylacetamide (DMA...

Several media for the storage of fresh semen from rainbow trout at 4 degrees C were evaluated. Semen was diluted with an equal volume of fluorocarbon, a 1:1 fluorocarbon:extender emulsion, a 2:1 fluorocarbon:extender emulsion, or with extender, or was stored undiluted. The semen was stored under moi...

Fifteen extenders were produced by adding dimethyl sulfoxide (DMSO) at 8, 10 or 12% of diluent volume to 5 diluents. All extenders were cooled to 4 degrees C. Pooled Atlantic salmon (Salmo salar ) semen with greater than 90% progressive motility was kept at 4 degrees C and added to each extender so ...

The objective was to determine the oxidative stability of Arctic char (Salvelinus alpinus) semen following dietary supplementation with lowbush blueberry (Vaccinium angustifolium) product, alpha-tocopherol, alpha-tocopherol+blueberry product, or alpha-tocopherol+astaxanthin. Sperm lipid peroxidation...

Five bulls with high levels of sperm-cell defects were evaluated for breeding soundness approx. once a month for several months. Considerable variation with time was observed in the levels of normal and abnormal sperm cells. These variations reflected only minimally on the breeding soundness ratings...

The accuracy of three in vitro methods for estimating the proportion of dead rainbow trout (Onchorhynchus mykiss ) spermatozoa was investigated. Motility rating, fluorometry using ethidium bromide, and lactate dehydrogenase (LDH) activity in seminal plasma were compared. Semen samples were prepared ...

Successful preservation of stallion spermatozoa is difficult because sperm damage occurs from lipid peroxidation during storage. We investigated the use of antioxidants with a standard skim milk extender and several gas environments during storage of stallion spermatozoa to reduce the harmful effect...