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The goal of this study was to obtain a complete description of the elastic modulus and hysteresis of the mantle tissue of the squid Loligo pealei over the entire functional region of the mantle. In addition, in vivo strains were measured to allow. calculations of the amount of energy stored and retu...
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Lampreys exhibit important structural and biochemical differences in their connective tissues relative to higher vertebrates. The lamprey ventral aorta wall, for example, is composed principally of microfibrils, not elastin and collagen as in higher vertebrates. Our working hypothesis was that this ...
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Marfan syndrome, a connective tissue disorder affecting the cardiovascular system, is caused by mutations of fibrillin-based microfibrils. These mutations often affect the calcium-binding domains, resulting in structural changes to the proteins. It is hypothesized that these Ca+2 binding sites regul...
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The physiological effects of repeated exposure to 9 mg l(-1) chloramine-T, a common aquaculture disinfectant for rainbow trout, were examined using a graded hypoxic challenge. Using an extracorporeal circulation, continuous measurements of blood PO2, PCO2 and pH were made and correlated with decreas...
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Immunoreactivity to growth hormone (GH), prolactin (PRL), and thyroid-stimulating hormone (TSH) was found in the adenohypophysis of the sea lamprey, Petromyzon marinus L., during its upstream migration, by immunoperoxidase techniques with antisera to mammalian pituitary hormones. Cross-reactivity to...
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The ultrastructural changes that take place in the ventral dermis along with the development of iridophores were examined in the anadromous sea lamprey, Petromyzon marinus, during metamorphosis. There is a disruption of all components of the ventral dermis and a reformation that results in a structu...
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Thyroxine (T4) concentrations in samples of fresh sera taken from fed and unfed larval (ammocoetes) and metamorphosing anadromous sea lamprey (Petromyzon marinus) were measured by means of radioimmunoassay. T4 levels in unfed ammocoetes after a 1-month period were not significantly different from th...
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The immunoreactivity of procollagen types I and III and of collagen type IV was detected in frozen sections of the growing apical end of rat incisor teeth by an indirect method making use of protein A. The sections were exposed to affinity-purified antibodies against these substances. The bound anti...
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Antibodies made against thyroglobulin (TG) were used in an immunocytochemical study for the light and electron microscopic localization of TG in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus, during its upstream migration. TG was found in the follicular lumen and in some colloi...
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Growth hormone, prolactin and somatostatin-like immunoreactivities were demonstrated in the brains of larval, young adult (parasitic) and upstream migrant adult sea lampreys, Petromyzon marinus, by means of immunoperoxidase techniques. Growth hormone (GH) and prolactin (PRL) were observed within sep...
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Two POMC-like pituitary prohormones proopiocortin (POC) and proopiomelanotropin (POM) have been characterized from adult sea lampreys (Petromyzon marinus). POC encodes a nasohypophysial factor (NHF), ACTH, an MSH, and beta-END; and POM encodes MSH-A, MSH-B, and beta-END. Two radiolabeled riboprobes,...
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The fine structure of mucocartilage, a tissue unique to larval lampreys, was examined in Petromyzon marinus L. This tissue is surrounded by a perichondrium of vascularized, dense connective tissue composed of fibroblasts, collagen fibrils, and elastic-like microfibrils, but it is avascular itself an...
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Immunohistochemical and ultrastructural methods were used to examine the distribution of elastin and the fine structure of the trabecular, nasal, branchial, and pericardial cartilages in the sea lamprey, Petromyzon marinus. The cells and matrix, as well as the overall organization of these component...
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A collagenous extracellular matrix was previously considered to be a requirement for classification of true cartilage. Data from the lamprey and hagfish now clearly indicate that both of these jawless craniates have extensive non-collagenous, yet cartilaginous endoskeletons. Non-collagenous cartilag...
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Thyroglobulin (TG) was localized in the endostyle of the anadromous sea lamprey, Petromyzon marinus L. by means of the unlabeled antibody peroxidase-antiperoxidase immunocytochemical method. TG was found localized on the apical surface and within the cytoplasm of type 2c and 3 cells and in some type...
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Immunoreactive fibronectin-like material was localized within tissues of agnathans (hagfishes and lampreys) by an immunoperoxidase technique. Fibronectin was detected in basement membranes and in loose and dense connective tissues throughout the agnathan body. A fibronectin-like component was also i...
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Corpora lutea from Sprague-Dawley rats that were orally administered 0.0 (control), 1.0, 10.0, and 100.0 mg/kg hexachlorobenzene (HCB) for 21 days were analyzed by electron microscopy. Granulosa lutein cells (GLC) from animals of the 10.0 mg group showed differences from the cells of animals that se...