Two 18-month-old bovine herpesvirus 1 (BHV1)-seronegative bulls were inoculated experimentally with BHV1 via their prepuces. Semen collected at intervals was examined by optimised virus isolation, dot-blot hybridisation and the polymerase chain reaction (PCR) for detection of BHV1, and the infection...

Infectious salmon anaemia virus (ISAV) is a new orthomyxovirus-like virus. Thirteen isolates of ISAV (11 from Canada, one from Norway and one from Scotland) were studied for their replication in the CHSE-214 cell line compared with that in the SHK-1 cell line. All isolates replicated in SHK-1 cells,...

There are currently four invasive tunicate species which are causing significant challenges to the aquaculture industry in Atlantic Canada: Ciona intestinalis, Styela clava, Botryllus schlosseri and Botrylloides violaceus. These tunicates foul mussel socks, aquaculture gear, boat hulls and wharves. ...

We have studied the replication of virus in tissues and development of lesions associated with infectious salmon anemia virus (ISAV) infection in Atlantic salmon using in situ hybridization (ISH) with a riboprobe targeting ISAV RNA segment 7 messenger RNA. Fish were infected with three ISAV isolates...

The host parasite system of the MSX parasite, Haplosporidium nelsoni, in the eastern oyster, Crassostrea virginica, poses many challenges for experimental study. The interactions that are involved in the development of the typically high prevalence and intensities of disease encountered in certain e...

Two viruses, infectious salmon anaemia (ISA) virus and a novel togavirus-like virus, were isolated from ISA disease outbreaks that were first reported as a new syndrome, haemorrhagic kidney syndrome (HKS) affecting farmed Atlantic salmon Salmo salar L. on the East coast of Canada. Laboratory confirm...