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The objective of this study was to describe the histopathologic changes observed in extrapituitary organs as well as the pituitary glands of horses with pituitary pars intermedia dysfunction (PPID). Adrenal gland, thyroid gland, liver, lung, kidney, heart, and pituitary gland from 32 horses with cli...
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Three cases of disseminated feline blastomycosis occurred in Wisconsin and northern Illinois. Two had unilateral uveitis and retinal detachment and were treated successfully with a combination of surgery and chemotherapy with amphotericin-B and ketoconazole. Another with central nervous system signs...
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Equine pituitary pars intermedia dysfunction (PPID) is a spontaneously occurring progressive disease affecting aged horses and ponies. The pathogenesis of PPID is poorly understood, but the available evidence supports a loss of dopaminergic inhibition of the melanotropes of the pars intermedia. Hors...
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Using single-locus microsatellite markers, higher levels of genetic variation were resolved than previously reported for northern pike (Esox lucius). 14 (AC)n.(TG)n microsatellite loci were isolated from a genomic library of northern pike. Primer pairs for the polymerase chain reaction were develope...
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Twenty-three smooth fox terriers with an inherited form of myasthenia gravis were studied. All dogs developed muscle weakness between 4 and 8 weeks of age. This weakness increased in severity with exercise and age. Muscle weakness was alleviated by the administration of a short acting cholinesterase...
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Conserving genetic diversity within and among populations of northern pike Esox lucius is important for maintaining their short-term fitness and long-term evolutionary potential. Northern pike have consistently shown low within-population variation, as detected by allozymes, mitochondrial DNA, and r...
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PCR primers that amplified 6 microsatellite loci in walleye were designed. The level of variation at these loci was examined in 186 adult walleye from Sioux Lake (Minnesota). The number of alleles per locus ranged from 4 to 10; the expected heterozygosity ranged from 0.4 to 0.78. Three of the 6 prim...
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A 12-year-old intact female miniature Schnauzer dog with a history of depression, anorexia and weakness including one episode of collapse was examined. Past pertinent history included recent repeated bouts of vomiting and the passage of black loosely formed faeces. The dog was moderately dehydrated ...
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Expression of the Caenorhabditis elegans Hox gene lin-39 begins in the embryo and continues in multiple larval cells, including the P cell lineages that generate ventral cord neurons (VCNs) and vulval precursor cells (VPCs). lin-39 is regulated by several factors and by Wnt and Ras signaling pathway...
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Late events in the cell-cell signalling pathway that controls the specification of vulva cell fates in C. elegans are characterized. The lin-31 gene acts downstream of the ras homolog let-60 and encodes a member of the HNF-3/fork head family of DNA-binding transcription factors. lin-31 regulates how...
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A 21-month-old intact male Saint Bernard was examined for a two-week history of hyperpnoea. Pleural effusion was confirmed radiographically, and fluid analysis was consistent with a modified transudate. Causes of pleural effusion such as heart failure, pyothorax, hypoalbuminaemia, chylothorax, lung ...
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The microsatellites were isolated from horse genomic DNA using a (GT)10 probe. They had 11-22 copies of a GT-AC dinucleotide repeat. PCR amplification of DNA from 13 reference stallions of diverse breeds from the International Equine Genome Mapping Workshop showed the presence of 2-7 alleles at the ...
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A genetic mosaic analysis procedure in which Caenorhabditis elegans mosaics are generated by spontaneous loss of an extrachromosomal array is described. This technique allows almost any C. elegans gene that can be used in germline transformation experiments to be used in mosaic analysis experiments....
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Traumatic detachment of the mitral valve from the annulus fibrosis occurred in a dog following blunt chest trauma. Euthanasia was elected approximately 7 months posttrauma due to refractory, chronic left heart failure. This is the first reported case of traumatic mitral valve rupture in a dog..
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The genetic population structure of yellow perch Perca flavescens was assessed, with a focus on spawning groups within Lake Michigan. Six microsatellite DNA loci were evaluated, which had heterozygosities ranging from 0.21 to 0.86 in samples from seven Lake Michigan locations and six others in the n...
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A microdissected pig genomic library was generated from the proximal half of the q arm of chromosome 6. Purified DNA was digested with restriction enzymes, ligated to oligonucleotide adaptors and amplified by PCR using primers complementary to the adaptor sequences. The purity of the amplified produ...
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Calcinosis circumscripta associated with polydioxanone suture material was diagnosed in two young dogs. In each case, the owner noticed a firm mass at the site of a previous surgical incision. Mineralization of soft tissues was visible radiographically. At excision, multiple chalky white nodular mas...
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Results demonstrated that xol-1 functions as an early developmental switch to set the choice of sexual fate and suggested that assessment of the X/A ratio occurs only early in embryogenesis to determine sex in Caenorhabditis elegans. Moreover, sdc-2, a gene that must be repressed by xol-1 to ensure ...
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4 newborn calves from different herds were examined because of recumbency and inability to rise. Abnormal physical findings were confined to the nervous and/or musculoskeletal systems. The calves were necropsied, and histological findings included multifocal lymphocytic myelitis, meningitis, and enc...
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We have documented an early life survival advantage by naturalized populations of anadromous rainbow trout Oncorhynchus mykiss over a more recently introduced hatchery population and outbreeding depression resulting from interbreeding between the two strains. We tested the hypothesis that offspring ...
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It has been shown previously that, in studies of hybrid populations there is a high probability of error in classifying individuals as parental, F1 or F2 on the basis of analysis of their genotypes. In the present work, the effects of the number of dominant loci examined on the probability of classi...
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The results showed that mpk-1 plays an important functional role as an activator in ras-mediated cell signalling in vivo in C. elegans..