Considering the poor facilities available for microbiologic diagnosis in some countries where B. abortus is a frequent cause of bovine abortion, a study was conducted to determine if isolation of B. abortus from an aborted bovine fetus could be predicted from a detailed histological study of the for...

Based on current literature which commonly associates bovine virus diarrhea virus and M. bovis with pneumonic pasteurellosis, an investigation was conducted into the effect of these 2 pathogens on the capacity of bovine lung to clear inhaled P. haemolytica. There was no significant effect (P > 0....

The purpose of this study was to determine if parainfluenza-3 virus in calves interferred with normal pulmonary bacterial clearance. Three groups (A, B and C) of 4 calves each were exposed to an aerosol of parainfluenza-3 virus. Three days later the 4 h pulmonary clearance of P. haemolytica was dete...

Since toxicity studies among different laboratories generally involve rats of different sex and age, this study was conducted to investigate the effect of sex, age and animal to animal variation in the activities of lactate dehydrogenase and alkaline phosphatase from bronchoalveolar lavage fluid, br...

This study was conducted to investigate the effect of sex, age, number of bronchoalveolar lavages and method of quantitation on the number of bronchoalveolar cells in rats. Forty Long Evans rats were divided into four age-sex subgroups of ten animals each. Nine consecutive bronchoalveolar lavages we...

This study was conducted to investigate the in vivo effect of a single intratracheal inoculation of Pasteurella haemolytica cytotoxin on the rat lung. Changes in the biochemical and cytological composition of bronchoalveolar lavage fluid were used to estimate the magnitude of pulmonary cell injury, ...

Ultrastructural and morphometric profiles of type-II pneumocytes (P-II) were investigated in rats killed 18 or 24 hours after a single intratracheal inoculation of bacterial (Escherichia coli) lipopolysaccharide (LPS). Inoculation with LPS induced pulmonary injury and inflammation, as measured by in...

Three, 5 or 7 days after inoculation with bovine viral diarrhea (BVD) virus (n = 12) or Mycoplasma bovis (n = 12), groups of calves were exposed to aerosols of Pasteurella haemolytica and were euthanatized 44 hours later. Histologic lesions in the lungs and the ratios of neutrophils to alveolar macr...

Fischer-344 rats were killed by exsanguination 1, 20, and 44 hr after a single 4-hr exposure to an atmosphere of 0, 10, 200, and 400 ppm of hydrogen sulfide (H2S). Alterations in the activities of lactate dehydrogenase and alkaline phosphatase, and cytomorphology of epithelial cells in fluids obtain...

Fischer-344 rats were exposed for 4 hours to 0, 14, 280, or 560 mg of hydrogen sulfide .cntdot. m-3 and killed 1, 18, or 44 hours later. We evaluated the nasal epithelial cells and determined the antaomic distribution of lesions. Inhalation of 560 mg of hydrogen sulfide .cntdot. m-3 induced necrosis...

The relationship between acute pulmonary cell injury and inflammatory response was investigated in rats killed 1, 3, and 7 days after intratracheal inoculation with bacterial lipopolysaccharide (LPS). Activities of lactate dehydrogenase (LDH) and alkaline phosphatase (AP) in bronchoalveolar lavage (...

The effect of vehicular saline solution volume on early lesions induced in rats by intratracheal administration of silica was evaluated. Seventy-two male Long-Evans rats were randomly assigned 6 each to 12 factorial groups (3 .times. 2 .times. 2): 3 doses of silica (0, 2.5, and 5 mg), 2 volumes of v...

The design of and the analysis of data from the Bruce County Beef Cattle Project, particularly the field study, are presented and discussed. A major value of the study may lie in its ability to relate laboratory findings to events in the field. The factor most strongly related to mortality was ratio...

Concentration-time interactions were investigated in young male and female Sprague-Dawley, Long Evans and Fischer-344 rats exposed to hydrogen sulphide for two,four or six hours. Higher concentrations caused more deaths, with no significant difference for duration of exposure. A significant sex effe...

The aim of this investigation was to do a comparative study on the deposition and clearance of inhaled bacteria between the lungs and tracheae of mice exposed to aerosols of bacteria. Mice were divided into 4 groups of 72 each and exposed to aerosols of P. haemolytica or S. aureus in 4 replicates. T...

38 Wistar rats with a mean weight of 177 g were divided in 5 groups. Rats were inoculated i.t. with 0.5 ml of fluid (made up to a constant volume using saline solution) containing 2.5 mg of silica, but a variable amount of amniotic fluid (0, 0.125, 0.250, 0.375 or 0.500 ml). The rats were killed 72 ...

Oxytocin is used to induce and control parturition, nevertheless, the increase of uterine contractions decreases blood flow and gaseous exchange through the womb predisposing to intra-partum mortality. The objective of the present study was to evaluate the effect of oxytocin on myometrial activity, ...

A dog adopted as a stray in Spain and then brought to Canada 4 years prior to presentation was evaluated for polyarthritis. An electrophoresis showed a marked polyclonal gammopathy and synovial smears contained leishmanial organisms within macrophages.

The meconium aspiration syndrome is an important cause of respiratory distress in newborn infants. Alveolar macrophages (AMs) provide a first line of defense in the lower respiratory tract against inhaled pathogens and particles such as meconium. In this study, we examined the effect of meconium on ...

A Clydesdale mare was examined for weight loss, inappetence, ptyalism, and dysphagia. The main abnormality revealed by serum biochemistry was a marked hyperglobulinemia, and protein electrophoresis revealed a monoclonal gammopathy in the gamma region. The urine was positive for Bence Jones proteins....

The ability of infectious bursal disease virus (IBDV) serotypes 1 and 2, and the role of VP4 of both serotypes as well as the capacity of three IBDV intermediate serotype 1-specific vaccine strains to induce apoptosis in a chicken B-lymphocyte cell line, DT40, were investigated using the TUNEL techn...

Experiments were undertaken to examine the chymotrypsin sensitivity and trypsin sensitivity of 13 avian reoviruses, and to determine if there was any correlation with pathogenicity of some chicken reoviruses. A wide variation in the degree of sensitivity of avian reoviruses to chymotrypsin and tryps...