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A lymphocyte stimulation test using antigens of Corynebacterium equi was used to compare the response of peripheral blood lymphocytes from foals with C equi pneumonia with those of clinically normal foals and adult horses. The test clearly distinguished infected foals from normal foals when tested i...
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The objective of this study was to determine the agreement between ELISA tests conducted using three O. ostertagia antigens: crude adult worm, larval stage 4 (L4) excretory/secretory (ES) and adult ES. This study was carried out on 289 Holstein cows from five herds in Prince Edward Island and one he...
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Angiostrongylus vasorum, French Heartworm, is a metastrongylid nematode infecting the pulmonary arteries and right heart of wild and domestic canids in various regions of the world. Infection in dogs can result in fatal cardiopulmonary disease. A single endemic focus of A. vasorum in North America o...
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A cross-sectional observational study was undertaken in Quebec dairy herds to evaluate regional and provincial prevalences of herds with gastrointestinal nematode (GIN) infections in lactating cows, and to determine risk factors associated with level of herd infection. The 5 most recently calved fir...
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Experimental infections with Piscirickettsia salmonis via intraperitoneal (IF), oral (PO) and gill (GS) routes were compared, and the importance of physical contact in the horizontal transmission of this organism was investigated. Atlantic salmon, Salmo salar L., under-yearling parr raised in fresh ...
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The general objective of this study was to evaluate a crude Ostertagia ostertagi antigen enzyme-linked immunosorbent assay (ELISA) for monitoring gastrointestinal parasites in lactating dairy cattle. A longitudinal study of gastrointestinal parasites in lactating dairy cows was carried out in 38 her...
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An indirect Ostertagia ostertagi ELISA was used in late lactation milk samples from cows in confined and semi-confined dairy herds to determine if it could predict milk production response after endectocide treatment at calving. Holstein cows from 9 dairy farms from Prince Edward Island (PEI), 5 fro...
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An indirect enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against Ostertagia ostertagi using a crude adult worm antigen was evaluated using serum and milk samples from adult cows, as well as from bulk tank milk. Within and between plate repeatabilities were determined. In...
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Immune response to fish microsporidia is still unknown and there are current research trying to elucidate the events involved in the immune response to this parasite. There is evidence suggesting the role of innate immune response and it is clear that adaptive immunity plays an essential part for el...
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Monthly bulk-tank milk samples were obtained from 415 Nova Scotia dairy herds in each of the months of July-September 1998 and tested using an indirect microtitre ELISA against a crude saline-extract, whole-worm Ostertagia ostertagi antigen. ELISA results (optical densities (ODs)) were consistent ac...
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The present study was carried out to evaluate the relationship between milk optical density ratios (ODRs) from an indirect Ostertagia ostertagi ELISA, total milk IgG levels and milk production and then establish a correction factor to adjust ODR. Five hundred and sixty composite milk samples collect...
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The objective of this study was to assess the stability of ELISA plates prepared with one of three blocking agents and used with one of two conjugates at various time intervals after preparation of the plates. Two of the blocking agents used were commercially available: one termed stabilgaurd (stab)...
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Using a randomized controlled trial design, a randomly allocated intervention group of 15 cows received a slow-release bolus that delivered 100 days of monensin. The negative control group of 15 cows received a placebo bolus that was identical to the monensin bolus, except without the monensin. Two ...
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The effects of selenium (Se) supplementation and source on equine immune function have not been extensively studied. This study examined the effects of oral Se supplementation and Se source on aspects of innate and adaptive immunity in horses. Fifteen horses were assigned to 1 of 3 groups (5 horses/...
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Background: The proportion of geriatric horses within the equine population has increased in the past decade, but there is limited information on the immune function of these animals. Hypothesis: Aged horses will have a lesser increase in serum antibody response to vaccination. Animals: Thirty-four ...
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The effect of organic and inorganic sources of selenium (Se) on measures of Se status of mares and their foals, Se concentrations of colostrum and milk, and indices of immune function of foals was studied. Twenty late-gestation Standardbred mares were randomly assigned to one of two groups. All mare...