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Serum antibody titers to bovine respiratory syncytial virus were determined for 559 cattle. Serum samples were obtained through the Minnesota State-Federal Brucellosis Laboratory and were collected over a 1-year period. Results of this study revealed an antibody prevalence of 65.5% to bovine respira...
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During a 19-month period, the agar gel immunodiffusion (AGID) test was used as an aid in differential diagnosis of paratuberculosis in 51 cattle with a history of chronic weight loss and/or chronic diarrhea. Thirty-three cattle were AGID test-positive for paratuberculosis. Twenty-eight cattle (87.5%...
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An indirect haemagglutination test (IHA) and ELISA technique were developed to detect antibodies against Eimeria tenella. The ELISA technique was relatively easy to perform, more sensitive than the IHA test, and needed only a fraction of the antigen required for IHA. The highest titers using ELISA w...
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Quercetin was able to reduce the cytotoxic effect of T-2 mycotoxin on cultured murine thymocytes. When given to mice immediately before challenge with T-2 mycotoxins, quercetin significantly reduced mortality.
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Pooled seminal plasma obtained from six bulls was tested for its immunosuppressive activities in vitro on blastogenesis of bovine lymphocytes. Lymphocyte stimulation assay and mixed lymphocyte reaction were used as test procedures. Concanavalin A and phytohemagglutinin were utilized in the lymphocyt...
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Topics discussed in this review include classification of viruses, virus structure, epidemiology of viral infections, cell-mediated and humoral response, viral pathogenicity, viral defences and the detection, treatment and prevention of viral diseases..
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Pasteurella haemolytica exerted a cytotoxic effect on bovine polymorphonuclear neutrophil leukocytes. This effect was less than that seen with cultured alveolar macrophages or peripheral blood monocytes. When alveolar macrophages were cultured with Pasteurella haemolytica, macrophages produced less ...
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The in vitro mitogen response of whole blood turkey lymphocytes to various concentrations of steroid hormones was evaluated. Corticosterone (COS) at concentrations between 1 and 80 ng/ml significantly suppressed the proliferative response (3H-thymidine incorporation) to phytohemagglutinin (PHA) and ...
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Six foals were inoculated intrabronchially with a suspension of Corynebacterium equi. Six weeks before this challenge, three foals were vaccinated with a C. equi bacterin. Three foals were unvaccinated controls. All foals developed a severe bronchopneumonia in the inoculated lung, indicating that va...
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The intragastric inoculation of a suspension of Corynebacterium equi on five consecutive days induced severe ulcerative colitis, typhlitis, and lymphadenitis of colonic and cecal nodes in two ponies necropsied three weeks after infection. No gross lesions were observed in two ponies necropsied ten d...
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Intradermal injection of phytohemagglutinin was used to evaluate the integrity of cell-mediated immunological reactions in Doberman puppies thought to be predisposed to demodicosis. Results indicate a statistically significant deficiency of cutaneous delayed response in these dogs when compared with...
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Cattle submitted to the University of Minnesota for surgical correction of left displaced abomasum (LDA) were examined for the in vitro phagocytic and bactericidal activities of their polymorphonuclear leukocytes (PMN). The PMN from cattle with LDA with or without concurrent infection had depressed ...
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Seminal plasma was determined to have immunosuppressive activities in vitro on Concanavalin A-induced blastogenesis of bovine lymphocytes. This immunosuppressive activity was isolated and characterized using a pool of seminal plasma from five bulls. The immunosuppressive activity was associated with...
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This study describes the isolation and partial characterization of a low molecular weight (approximately 14 kDa), cadmium-binding protein from rainbow trout (Onchorynchus mykiss) liver. Rainbow trout were injected intraperitoneally with 3.5 mg/kg cadmium chloride (total body dose) twice weekly for 3...
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An anionic detergent, sodiumdodecylbenzenesulphonate, had an adjuvant effect upon aerosol allergic sensitization with subtilopeptidase A, a proteolytic enzyme of Bacillus subtilis. Both local and systemic antibody responses were accelerated and prolonged by use of the detergent. This adjuvant effect...
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The effect of vitamin E injections on immune responses of calves was investigated. Treatments were: 0, 900, 1800 and 2700 IU of D-alpha-tocopherol given by injection starting at birth and then a 3 wk interval until the age of 12 wk. Plasma vitamin E levels were significantly higher for supplemented ...
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Bovine respiratory syncytial virus (BRSV) was the viral agent most commonly identified in 14 epizootics of pneumonia in dairy calves. A microtiter serum-virus neutralization test proved to be the best means of identifying involvement of BRSV; seroconversion (fourfold or greater rise in titer) was de...
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Two experiments were conducted to evaluate the effects of oral supplementation of vitamin C and vitamin E (alpha-tocopherol), alone and in combination, on immune responses of calves. In Experiment 1, 18 Holstein newborn female calves were supplemented with 0, 1, and 2 g/d of vitamin C from birth to ...
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In Ireland sprinkler irrigation applied to black currant bushes at the rate of 120 gal. per min. during the nights in April and May when freezing temperatures were recorded gave protection against frost. Yields were 58 cwt./acre compared to 31 cwt./acre in the unsprinkled control..
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Six foals were inoculated intrabronchially with a suspension of C. equi. Six weeks before this challenge, three foals were vaccinated with a C. equi bacterin. Three foals were unvaccinated controls. All foals developed a severe bronchopneumonia in the inoculated lung, indicating that vaccination was...
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Bovine fetal lung tissue culture cells were infected with bovine viral diarrhea virus. The cells released substances into the supernatant fluid that suppressed the proliferative response of bovine peripheral blood mononuclear cells which had been stimulated with concanavalin A. Activity could be fou...