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This paper (only an abstract of which is given) was presented at the Sixteenth Annual Meeting of the Society of Nematologists held at East Lansing, Michigan, from 16 to 19 August 1977. An account is given of studies carried out in Canada on the effect of parasitism by the mermithid nematode Neomesom...
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R. culicivorax juveniles were dissected out of Aedes aegypti larvae 7 days after infection and incubated under controlled conditions in isotonic saline containing a 14C-labelled fatty acid (palmitic acid), monoacylglycerol (glycerol monoolein), or triacylglycerol (glycerol tripalmitate) nutrient sou...
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Eggs (0-24 h old and 48-72 h old) of Choristoneura fumiferana were treated topically with fenoxycarb dissolved in acetone. Only 25% of treated eggs hatched, compared to 90% of control eggs. Survival of larvae from treated eggs to induction of diapause in the 2nd instar, and of diapausing larvae, was...
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Isocitrate lyase and malate synthetase, key enzymes of the glyoxylate cycle, were present in postparasites of R. culicivorax. Specific activities of enzymes were higher in adult postparasites than in newly emerged juveniles. Isocitrate lyase had a well-defined pH optimum (7.5), whereas malate synthe...
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The effect of 7 juvenile hormone analogues on the development of larvae of Choristoneura fumiferana was studied in the laboratory at 23 degrees C, LD 16:8 and 65% RH by topical application of the compounds to early 6th-instar larvae. Fenoxycarb showed the highest potential for control, with an LC50 ...
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The haemolymph of larvae of Prosimulium mixtum Syme & Davies/P. fuscum Syme & Davies and Simulium venustum Say parasitised by the mermithid nematode Neomesomermis flumenalis was compared with that of unparasitised hosts with respect to protein, amino acid and carbohydrate composition. The me...
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Early 6th-instar larvae of Choristoneura fumiferana were topically treated with the juvenile hormone analogue fenoxycarb, and fat bodies were isolated at varying times post-treatment for metabolic studies. Fenoxycarb impaired lipid synthesis by the fat bodies, suppressing both the synthesis of fatty...
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The nervous system of the mermithid nematode Romanomermis culicivorax was investigated at different developmental stages for the distribution of catecholamine(s). The catecholamines nervous system increased in complexity during development..
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Two bacterial symbionts of entomopathogenic nematodes, one of which originated from Texas, U.S.A., and the other from Newfoundland, Canada, were characterized phenotypically. These strains belonged to the genus Xenorhabdus. The Newfoundland (NF) strain was shown to be X. bovienii but the Texas (TX) ...
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A method of collecting postparasitic Neomesomermis flumenalis from larval Prosimulium mixtum/fuscum and Simulium venustum is described. The larval blackflies were collected by sweeping them with paint brushes from rocks into buckets of stream water. The larvae were obtained free of debris by placing...
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Analysis of haemolymph samples from field-collected larvae of Simulium venustum Say, S. vittatum Zett. and either Prosimulium mixtum Syme & Davies or P. fuscum Syme & Davies or a mixture of the two showed the haemolymph of all species to be essentially similar with respect to amino-acid pool...
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In the first part of this study, generation times relative to temperature, together with cardinal and conceptual temperatures, were determined for four strains of Xenorhabdus bacteria that represented three geographically distinct species. The data showed that the NF strain of Xenorhabdus bovienii, ...
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Four isolates (= 3 species) of steinernematids were propagated at 25, 20 and when possible, 15 and 10 degrees C for 2 years, then their capacities for tolerating high temperatures and freezing determined. In all isolates, the temperature at which 50% of the infective juveniles died (LT50) increased ...
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Histological observations were made on larvae of Aedes aegypti (L.) parasitised for 2, 4 or 6 days by Reesimermis nielseni. Little difference was detected between the tissues of uninfected and parasitised larvae 4 days after infection, at which time most hosts were in the early fourth instar and the...
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R. culicivorax larvae, dissected out of Aedes aegypti larvae 7 days after infection, were incubated under controlled conditions in isotonic saline containing 14C-U-palmitic acid to investigate the nature of the transport mechanism(s) used by the nematode for transcuticular uptake of palmitic acid. N...
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A steinernematid nematode was isolated from soil samples collected near St. John's, Newfoundland, Canada. On the basis of its morphometry and RFLPs in ribosomal DNA spacer, it was designated as a new strain, NF, of Steinernema feltiae. Cellulose acetate electrophoresis was used to separate isozymes ...
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The effects of methoprene on post-feeding larvae of Delia radicum was investigated. Treatment of larvae with methoprene at 1 micro g did not affect the capacity of the insect to pupate, but suppressed eclosion to the adult stage. The concentration of haemolymph trehalose was significantly decreased ...
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The nutrient composition of postparasitic females (newly emerged juveniles, newly moulted adults, and spent adults) and eggs of Romanomermis culicivorax was studied. Postparasites were obtained from experimentally infected larvae of the mosquito Aedes aegypti. Throughout postparasitic development, p...
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Exposure of early 4th-instar larvae of Aedes aegypti (L.) to the juvenile hormone analogue methoprene (Altosid) significantly increased the concentration of carbohydrates in the haemolymph of late 4th-instar larvae and reduced the haemolymph carbohydrate concentration of 24-h-old pupae relative to c...
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The relative concentrations of catecholamine in the nervous system of Romanomermis culicivorax were measured under different experimental conditions by a glyoxylic acid-induced fluorescence procedure. A greater concentration of catecholamine was recorded in the nervous system of adult males and fema...
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Sixth-instar larvae of Choristoneura fumiferana were treated with LD50 concn of the juvenile hormone analogues fenoxycarb and methoprene. Fenoxycarb caused an initial decrease, then an increase, in haemolymph concn. Fat-body glycogen was depleted initially, then relatively increased in concn towards...