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Four hundred eighty mature marine mussels (Mytilus edulis) were collected in late April from a mussel lease in an estuary in Prince Edward Island, Canada. Squash preparations made of reproductive gland (mantle) tissue of each mussel were stained with Wright-Giemsa stain and were examined by one inve...
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Blue mussel Mytilus edulis aquaculture lines and associated gear provide habitat for sessile and mobile epifaunal fouling organisms. Due to food limitations and substrate space, these species are likely to interact among themselves and with mussels. In some areas of Prince Edward Island and elsewher...
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Since all retroviruses possess reverse transcriptase (RT) enzyme, reverse transcriptase activity has been the main supportive evidence of retroviral etiology of haemic neoplasia (HN) in soft shell clams, Mya arenaria. The objective of the present study was to search for a putative retrovirus in vari...
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Mussel (Mytilus edulis) culture in Atlantic Canada utilizes wild seed harvested from spat collection sites. The economics associated with wild-caught seed have proven to be more favourable than with hatchery-produced seed. This is not, however, the case in all areas. In Washington state, seed used f...
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This report describes the first 4 years of an experimental rotation in ecosystem health offered to senior veterinary students in Canada. Faculty from the 4 Canadian veterinary colleges collaborated in offering the rotation once annually at one of the colleges. The first rotation was held in Guelph, ...
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Invasive tunicates are creating costly fouling problems to the mussel aquaculture industry in many regions including Prince Edward Island, Canada. These invasive tunicates are also posing a threat to the ecosystem integrity of native species. There are currently four invasive tunicate species presen...
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The toxicity of BMS-182248, an immunoglobulin (cBR96)-cytotoxic drug (doxorubicin) conjugate, was investigated in Sprague-Dawley rats at single intravenous doses of 508, 1,200, and 2,550 mg/m(2) (conjugated doxorubicin doses of 14.7, 34.8, and 74 mg/m(2), respectively) and compared to that obtained ...
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The development of effective mitigation techniques against Botryllus schlosseri and Botrylloides violaceus colonizing blue mussel
aquaculture operations has not been well studied. The objectives of our research were to determine the efficacy of using pressurized seawater
in the mitigation ...
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Oncostatin M (OM) is a member of the interleukin-6 (IL-6) cytokine subfamily. The binding of OM to its receptor initiates signal transduction through JAK-signal transducers and activators of transcription (STAT) pathways and activates transcription activators through mitogen-activated protein (MAP) ...
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Resistance development to parasiticides has been a problem in both terrestrial and aquatic environments. A major challenge exists in early detection of resistance emergence due to the low frequency at which resistant organisms can occur initially within a population and the difficulty in detecting t...
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The study of protein function in neurons has been hindered by the lack of highly efficient, nontoxic methods of inducing RNA interference in such cells. Here we show that application of synthetic small interfering RNA( siRNA) linked to the vector peptide Penetratin1 results in rapid, highly efficien...
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BR96 sFv-PE40, a recombinant DNA-derived fusion protein composed of the heavy- and light-chain variable region domains of the monoclonal antibody BR96 and the translocation and catalytic domains of Pseudomonas exotoxin A, is being developed for the treatment of solid tumors expressing cell surface L...
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A colorimetric method (glucose oxidase/peroxidase) for indirect measurement of glycogen concentrations in tissue homogenates of marine mussels (Mytilus edulis) was evaluated. This method uses a conversion of glycogen to glucose by amyloglucosidase. Varying the buffer pH (4.5, 5.0; 5.5) and the amylo...
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A colorimetric method for indirect measurement of glycogen concentrations in tissue homogenates of eastern oysters (Crassostrea virginica) was evaluated. This method uses a conversion of glycogen to glucose by amyloglucosidase. The procedure was optimized Fur extracting buffer pH (5.0) and amylogluc...
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In 1999, examination of soft-shell clam (Mya arenaria) mortalities at an experimental lease on Prince Edward Island (PEI) revealed a 95% prevalence of advanced haemic neoplasia (leukaemia). Low prevalences of this condition had been documented previously in soft-shell clams from Nova Scotia and the ...
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The first confirmed report of the invasive solitary ascidian Styela clava (clubbed tunicate) in the blue mussel (Mytilus edulis)
production area of March Water in Malpeque Bay, Prince Edward Island (PEI), occurred in September of 2002. Mussel farms in
eastern PEI waters have been challenge...
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Spatial growth variation of experimentally cultured blue mussels Mytilus edulis in Tracadie Bay, Prince Edward Island, Canada, was consistent in both years of a 2 yr study. The spatial pattern displayed reduced tissue growth along a gradient of decreasing tidal exchange from the inlet mouth to the i...
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Invasive species such as ascidians have negative effects on aquaculture operations worldwide. Prince Edward Island, Canada, in particular has seen high fouling levels of non-native tunicates including the colonials Botryllus schlosseri and Botrylloides violaceus. Previous research indicated that hig...