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Effective methods of traceability are urgently required for use in research as well as in different types of aquaculture operations and to control trade in aquatic animals and products. In regard to the marking of fish, many different tagging methods have been described and the method to be used dep...
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The protozoan parasite Perkinsus atlanticus (Azevedo, 1989) causes severe losses among cultured clams, Ruditapes decussatus. This parasite is routinely diagnosed by means of histology or incubation of gills in fluid thioglycollate medium. However, in order to develop models of experimental reproduct...
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The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains ...
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One of limiting factors in shrimp culture is the mortality at the larval stage. Often bacterial diseases are suspected to be the origin of this damage. This work concerns the study of relations between microorganisms (bacteria and Vibrio) and peneids. Observations induced a new approach to bacteriol...
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Original Abstract: Le protozoaire parasite Marteilia refringens est responsable d'importantes mortalites au sein des elevages d'huitres plates Ostrea edulis, et a participe a la disparition de 90% de la production depuis les annees 60. Une gestion optimale des risques de contamination des huitres pl...
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A nested polymerase chain reaction (PCR) has been developed for the diagnosis of Marteilia refringens in mussels (Mytilus galloprovincialis) from Galicia (NW Spain). Specific primers were used in two consecutive amplification reactions, that were able to detect 15 fg of Marteilia DNA. The nested PCR...
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The French mollusc production is mainly based on the Pacific cupped oyster, Crassostrea gigas. Since 1991, outbreaks of mass mortality of juveniles are reported during the summer period. These outbreaks are a major concern of oyster industry. Several studies have established given bacterial strains ...
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In 1999, the world aquaculture production of molluscs was estimated at 9.4 million tonnes which represents approximately a 23% contribution to the global aquaculture production. Aquaculture of molluscs has been growing at an average annual rate of 11% over the past decades, although some activities ...
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In Argentina, especially in San Matias Gulf, oyster culture is a recent activity. Culture of native flat oyster, Ostrea puelchana (d'Orbigny, 1842), was particularly promising until an abnormal mortality occurred in 1996. Preliminary diagnosis, based on histology, revealed the presence of Bonamia-li...
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Disseminated neoplasia (DN) is a disorder referred to as hemic neoplasia (HN) in the soft-shell clam Mya arenaria. Traditionally, diagnosis is performed by hematocytology or histology. The intensity of the disease is generally given as the percentage of transformed neoplastic cells Out of total numb...
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A parasite of the dredge oyster Ostrea chilensis Philippi, previously known as Bonamia sp., was recently described and named Bonamia exitiosus, based on ultrastructural and molecular evidence. The rules governing the names of organisms require correspondence of gender, and the name must be changed f...
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The flat oyster Ostrea chilensis is susceptible to infection with two microcells, Bonamia exitiosa and Bonamia ostreae. A haemocytic parasite very similar to Bonamia species has been previously reported in two of 167 Chilean oysters sampled from Chiloe Island in 1996-1997. In order to characterise t...
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Diagnosis of haemic neoplasia (HN) in the soft shell clam, Mya arenaria, is often achieved by hematocytology and histology. Since neoplastic cells display tetraploid DNA contents, haemocyte cell cycle analysis was developed for use as a diagnosis tool. The aim of this study was to assess the applica...
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The molecular mechanisms by which haemocytes of clams are transformed in the course of haemic neoplasia remain by far unknown. The aim of this study was to quantify the expression of p53/p73 and mortalin genes, in relation with the ploidy status of clam haemocytes and to correlate the p53 expression...
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Gene expression Studies have opened a tremendous field of investigation in biological research over the last decades. Expression of genes is most frequently quantified by real time PCR (RT-qPCR), as this method has proven to be highly sensitive. One of the critical steps, however, in comparing trans...
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Sample-size calculations in the context of surveys aimed at substantiating freedom from infection have been commonly undertaken on terrestrial animals over recent years, but not on aquatic animals. A recent model developed by Audige and Beckett in 1999 can be used to plan and assess animal health su...
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The life history of Marteilia refringens was investigated. The infectivity of purified early and late stages of M. refringens to oysters (Ostrea edulis) was tested in the laboratory using different routes of inoculation. Naturally infected oysters were monitored in experimental tanks to study infect...
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This supplement presents the results of a survey on Mediterranean aquaculture diagnostic laboratories, which has been implemented by the Mediterranean Institute of Zaragoza (IAMZ) in collaboration with the FAO Fisheries Department. The aim of the survey is to acquire greater knowledge on the Mediter...
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Bonamia ostreae is a protozoan parasite of the flat oyster, Ostrea edulis, which has caused significant loss of oysters in Europe over the last decade. B. ostreae was purified from infected flat oysters and DNA was extracted. The nearly complete small subunit rDNA gene of B. ostreae was amplified us...